Method for large-scale breeding non-toxic myzus persicae
A technology of tobacco aphids and tobacco, applied in the field of large-scale breeding of non-toxic aphids, can solve the problems of high cost of elimination, difficult sorting, hidden disease and poisoning, and achieve the effect of reducing risks
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Embodiment 1
[0017] Coker176 carrying the N gene as the anti-TMV parent and NC55 carrying the va gene locus as the anti-PVY parent were crossed by conventional methods to obtain F 1 generation. The F 1 The genotype was NnVava, showing resistance to TMV but sensitivity to PVY. f 1 Selfing by conventional method, get F 2 groups, in F 2 In the population, through mixed inoculation of TMV and PVY, or detection of molecular markers of N gene and va gene, screen single plants with double antibodies against TMV and PVY, that is, single plants with genotype NNvava or genotype Nnvava. Individual plants with genotype NNvava were screened by test cross. Among the individual plants whose genotype is NNvava, the individual plants with vigorous growth were selected, and the seeds were selfed to obtain double-resistant tobacco materials (RTP) with stable resistance to TMV and PVY. Further artificially inoculate TMV and PVY viruses, verify the resistance to TMV and PVY, and breed aphids by breeding ...
Embodiment 2
[0020] Example 1 was repeated with the following differences: CV87 carrying the N gene was used as the anti-TMV parent, and NC55 carrying the va gene locus was used as the anti-PVY parent.
Embodiment 3
[0022] Repeat Example 1 with the following differences: CV87 carrying the N gene is used as the anti-TMV parent, and VSCR carrying the va gene locus is used as the anti-PVY parent.
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