Method for large-scale breeding non-toxic myzus persicae

A technology of tobacco aphids and tobacco, applied in the field of large-scale breeding of non-toxic aphids, can solve the problems of high cost of elimination, difficult sorting, hidden disease and poisoning, and achieve the effect of reducing risks

Active Publication Date: 2013-04-03
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as high cost, cumbersome steps, difficult implementation, and difficulty in achieving high efficiency.
The main problems are reflected in the following aspects: 1) The individuals of Aphididae and aphids are small but the populations are huge, so it is difficult to sort them. The separation method of Aphididae adult bees and winged aphids can still be completely separated
2) As a result of long-term evolution, the parasitism rate of nymphs cannot reach 100% in large-scale breeding sheds
3) The prevention and control of common plant virus diseases is difficult
In frequent operations and repeated use of net rooms or greenhouses, TMV and PVY and other serious plant viruses have the characteristics of multiple sources of initial infection, easy transmission, fast transmission speed, latent infection, and poor control effect of chemicals.
Once a shed is poisonous, it will be costly and difficult to completely eliminate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Coker176 carrying the N gene as the anti-TMV parent and NC55 carrying the va gene locus as the anti-PVY parent were crossed by conventional methods to obtain F 1 generation. The F 1 The genotype was NnVava, showing resistance to TMV but sensitivity to PVY. f 1 Selfing by conventional method, get F 2 groups, in F 2 In the population, through mixed inoculation of TMV and PVY, or detection of molecular markers of N gene and va gene, screen single plants with double antibodies against TMV and PVY, that is, single plants with genotype NNvava or genotype Nnvava. Individual plants with genotype NNvava were screened by test cross. Among the individual plants whose genotype is NNvava, the individual plants with vigorous growth were selected, and the seeds were selfed to obtain double-resistant tobacco materials (RTP) with stable resistance to TMV and PVY. Further artificially inoculate TMV and PVY viruses, verify the resistance to TMV and PVY, and breed aphids by breeding ...

Embodiment 2

[0020] Example 1 was repeated with the following differences: CV87 carrying the N gene was used as the anti-TMV parent, and NC55 carrying the va gene locus was used as the anti-PVY parent.

Embodiment 3

[0022] Repeat Example 1 with the following differences: CV87 carrying the N gene is used as the anti-TMV parent, and VSCR carrying the va gene locus is used as the anti-PVY parent.

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PUM

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Abstract

The invention discloses a method large-scale breeding non-toxic myzus persicae. A tobacco material resistant to TMV (Tobacco Mosaic Virus) and PVY (Potato Virus Y) is used as a breeding host of aphids. After seedling growing or potting or transplanting, the aphids are artificially bred in a net house or a greenhouse, and a double-resistant strain with a high aphid breeding efficiency is selected to be used for breeding non-toxic aphids. The method adopts the tobacco material resistant to the TMV and the PVY to breed the aphids and can effectively reduce the potential risk of the aphids carrying virus diseases under the condition that the current aphid parasitoid breeding process and the cost are not increased. The method has the characteristics of simplicity, convenience and easiness in operation, economy, reliability and the like and can promote popularization and application of aphid parasitoid control crop aphids.

Description

Technical field: [0001] The invention belongs to the biological control of aphids in the field of plant protection, in particular to a method for large-scale propagation of non-toxic aphids. Background technique: [0002] Aphidius gifuensis is an endoparasitic wasp specialized in parasitizing aphids, and is an important parasitic natural enemy of tobacco field Aphidius gifuensis. Aphids aphids is widely used in biological control of aphids. The main steps of preventing and controlling aphids by the aphids include: planting plants, raising aphids on the plants, using the aphids raised on the plants, and raising the aphids. The purpose of preventing and controlling aphids is achieved by artificially releasing and rearing the aphids in the field to parasitize the aphids on the crops in the field. Aphididae rearing consists of two main phases: 1) seed aphids and overwintering of seedling bees; 2) commercial Aphididae production and release phases. In small sheds or large shed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01H1/02
Inventor 刘勇吴兴富朱艰李梅云许美玲卢秀萍李永平杨华兵
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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