Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants

Abstract

The invention relates to the field of plant propagation, and particularly provides an industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants, which comprises the following steps: (1) treating branches of Changbai Mountain rhododendron plants, and taking the treated branches as explants for later use; (2) preparing a culture medium for enabling stems to take root and axillary buds to germinate and grow simultaneously: adding kinetin KT (0.03-0.010 mg.L<-1>), indolebutyric acid IBA (0.10-0.45 mg.L<-1>), naphthylacetic acid NAA (0.06-0.13 mg.L<-1>), gibberellin GA3 (1.40-2.60 mg.L<-1>), agar powder (7.8 g.L<-1>) and sucrose (10.0 g.L<-1>) into a basic culture medium, and regulating the pH value to 5.6; and culturing under the conditions that the illumination period is 9 h.d<-1>, the illumination intensity is 1000 lx and the temperature is 23+ / -2 DEG C; (3) performing mycorrhizal treatment on tissue culture seedlings; and (4) culturing the tissue culture seedlings, and propagating to realize the industrial production of mycorrhizal seedlings of rhododendrons. According to the invention, the rhododendron plants can be subjected to mycorrhizal treatment at a high survival rate and can quickly propagate, thus finally realizing the industrial production of mycorrhizal seedlings of 9 rhododendrons on Changbai Mountain.

Description

[0001] [0002] technical field [0003] The invention relates to the field of plant propagation, that is, an industrialized method for mycorrhized seedlings of rhododendrons in Changbai Mountain. Background technique [0004] In the prior art, Rhododendron plants in Changbai Mountains include Rhododendron cowhide ( Rhododendron chrysanthum Pall.), short-fruited cuckoo ( Rhododendron brachycarpum D. Don), Zhaobai Rhododendron ( Rhododendron micranthum Turcz.), Rhododendron microphylla ( Rhododendron parvifolium Adams.), Rhododendron ( Rhododendron schlippenbachii Maxim.), Xing'an Rhododendron ( Rhododendron dauricum L.), Red Rhododendron ( Rhododendron mucronulatum Turcz.), felt cuckoo ( Rhododendron confertissimum Nakai) and rhododendron ( Rhododendron redowskianum Maxim.) and other 9 species, all of which are key protected plants in Jilin Province. Among them, Rhododendron spp. and Rhododendron bracts are precious and rare plants under the third-level...

AI Technical Summary

Problems solved by technology

These nine kinds of rhododendrons have small seeds, low germination rate, and extremely low survival rate of seedlings formed after germination; cutting propagation requires a large number of wild rhododendron branches, which greatly damages wild resources, and the rooting rate and transplanting survival rate are extremely low; and The use of tissue culture technology for propagation has the advantages of large annual output of test-tube seedlings and fast speed, but there are still problems such as low survival rate of hardened seedlings and transplanting, slow growth, etc., which leads to certain restrictions on popularization and application