Fusarium oxysporum bitter gourd specialized molecular marker and application thereof

Abstract

The invention discloses a fusarium oxysporum bitter gourd specialized RAPD (random amplified polymorphic DNA) marker (SEQ.ID.No.1), an SCAR (sequence characterized amplified region) marker (SEQ.ID.No.2) obtained by transforming the RAPD marker and application of the molecular markers in pathogenic bacteria identification and pathogenetic tissue detection. The molecular marker disclosed by the invention has fusarium oxysporum bitter gourd specialized strain specificity, consistency and stability, can distinguish difference between the specialized strain and other specialized pathogenic bacteria, can identify fusarium oxysporum bitter gourd specialized pathogens and plays an important role in identification of the fusarium oxysporum bitter gourd specialized strain, so that a foundation is laid for building a special, quick, high-sensitivity, stable and reliable molecular detection technical system for fusarium oxysporum bitter gourd specialized pathogenic bacteria. By applying the molecular marker disclosed by the invention, the defects that workload is high and cycle is long in the conventional fusarium oxysporum bitter gourd specialized pathogenic bacterium identifying and distinguishing process can be overcome, and quick and accurate identification and detection on pathogens are realized.

Description

technical field [0001] The present invention belongs to the technical field of molecular markers, and in particular relates to molecular markers of Fusarium oxysporum balsam pear specialization and its application, specifically RAPD markers of Fusarium oxysporum balsam pear specialization, SCAR markers transformed from the markers and these molecules Application of markers in identification of pathogenic bacteria and detection of diseased tissues. Background technique [0002] Bitter melon wilt caused by Fusarium oxysporum Schl.f.sp. Momordicae Sun & Huang (FOM) is a serious fungal disease that has spread throughout the main producing areas of Asian bitter melon such as Countries such as the Philippines, Japan, and India have serious outbreaks in provinces (regions) such as Guangxi, Guangdong, Hunan, Fujian, Zhejiang, Jiangxi, and Taiwan, resulting in huge losses of bitter melon production. Fusarium oxysporum monotype and other 6 known pathogens of Fusarium oxysporum monoty...

AI Technical Summary

Problems solved by technology

[0002] Bitter melon wilt caused by Fusarium oxysporum Schl.f.sp. Momordicae Sun & Huang (FOM) is a serious fungal disease that has spread throughout the main producing areas of Asian bitter melon such as The Philippines, Japan, India and other countries have serious outbreaks in provinces (regions) such as Guangxi, Guangdong, Hunan, Fujian, Zhejiang, Jiangxi and Taiwan, resulting in huge losses of bitter gourd production.

Fusarium oxysporum monotype and other 6 known pathogens of Fusarium oxysporum monotype that cause Fusarium wilt of Cucurbitaceae melon crops are very close in phenotype and difficult to distinguish, mainly relying on the pathogenicity of special hosts The traditional inoculation identification method takes a long period of time and is time-consuming and labor-intensive.

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