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Application of nickel aluminum layered double metal hydroxide modified electrode to measurement of uric acid

A layered bimetallic and modified electrode technology, applied in the field of electrochemical analysis, to achieve the effects of simple instrument requirements, low detection limit and high stability

Inactive Publication Date: 2013-04-24
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Although the enzymatic method has good selectivity, it is expensive, which greatly limits its application range

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  • Application of nickel aluminum layered double metal hydroxide modified electrode to measurement of uric acid

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preparation example Construction

[0033] A 0.3% chitosan-acetic acid solution was prepared and diluted five times before use. Weigh 2 mg of NiAl-LDH / G composite material and add it to 1 ml of the above-mentioned diluted solution, mix ultrasonically for 30 min at room temperature, and apply 10 μl of the above-mentioned ultrasonically dispersed solution on the surface of a clean glassy carbon electrode. Dry under infrared lamp conditions until the solvent is completely volatilized, and a film containing NiAl-LDH / G composite is formed on the surface of the glassy carbon electrode, that is, a NiAl-LDH / G composite electrode is obtained. NiAl-LDH membrane electrodes were prepared by the same method for comparative experiments.

[0034] App detection:

[0035] Investigate the electrocatalytic performance of NiAl-LDH / G composite:

[0036] At room temperature (20±1°C), CHI660D electrochemical workstation was used for cyclic voltammetry test. The test system was uric acid standard solution prepared in PBS with differe...

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Abstract

The invention discloses an application of a nickel aluminum layered double metal hydroxide modified electrode to the measurement of uric acid. The application comprises the following steps: (1) preparing the nickel aluminum layered double metal hydroxide modified electrode NiAl-LDH / G; (2) inspecting the electric catalytic performance of a NiAl-LDH / G compound; (3) inspecting the DPV (differential pulse voltammetry) behaviors of UA (uric acid) on different modified electrodes; (4) quantitatively detecting UA; and (5) inspecting the repeatability, the stability and the interference test of the electrode. Compared with the prior art, the application has the advantages as follows: compared with other conventional detection modes, the detection method is simple and quick, the sensitivity is high, the detection limit is low, and a reaction system is nonhazardous; the method is high in stability, low in requirement on instruments and easy to popularize and use; and the method also has the characteristics of being simple, convenient and quick to operate.

Description

Technical field: [0001] The invention belongs to the technical field of electrochemical analysis, and mainly relates to the application of NiAl-LDH / G modified electrode NiAl-LDH / G in the determination of uric acid. Background technique: [0002] The biomolecule uric acid (UA) is the final product of purine metabolism in the human body. It is mainly decomposed by enzymes to form allantoin and is freely excreted in the urine. Changes in the content of uric acid in the human body can fully reflect the functional status of the body's metabolism and immunity. At the same time, its high content can be related to many diseases, such as cardiovascular disease, gout, diabetes, kidney disease, heart disease, etc. Research by Tomita et al. found that the blood uric acid level (6.18 mg / dL) in ex-smokers was 0.2 mg / dL higher than that in current smokers. Therefore, it is of great significance to establish a method for the highly sensitive and accurate quantitative detection of UA in h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 侯宏卫田永峰张小涛刘彤陈欢韩书磊胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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