Primers and method for purity identification of hybrid seeds of Chinese pumpkin 'Guangmi NO.1'

Abstract

The invention discloses primers and a method for purity identification of hybrid seeds of Chinese pumpkin 'Guangmi NO.1'. The primers are VRISSRPMK8-F:5'-TAATTAAGGCGTGAAATTGG-3' and VRISSRPMK8-R:5'-GAAAAGAAACATAAACAGGGC-3'. The identification method is as follows: 1) extracting a genome DNA (deoxyribonucleic acid) of a pumpkin seedling; 2) carrying out PCR (polymerase chain reaction) amplification by taking the genome DNA of the pumpkin as a template and using the SSR primers VRISSRPMK8; 3) carrying out gel electrophoresis on the amplified product; and 4) analyzing the electrophoresis result, wherein only the single plant simultaneously having parent specific bands is a real hybrid, and the plant lacking of any one band is marked as false hybrid. The primers and the method can be used for effectively distinguishing the hybrid seed of the 'Guangmi NO.1' from the female parent and the male parent, and can identify the purity of the hybrid seeds rapidly and accurately. The method has the advantages of being fast and accurate, low in cost and simple in operation, and the like.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for quick detection of pure-hybrids from both females or males that produce them. It also provides technical benefits such as fast response time, accurate results with lower costs compared to current techniques like identifying individual seals by coloring them visually before they mature into edible crops.

Problems solved by technology

Technically speaking, this patented technical problem addressed in this patents relates to testing pure or unclean seals produced through breeding methods that involve removing unwanted parts like stamens after fertilization with spermatozoa collected separately at specific locations within their own fields. Current techniques require multiple steps such as collecting ovules from each plot, isolating them manually, then analyzing these isolated material to determine if any contamination has been introduced into the resulting crops. These procedures take up too much time and cause environmental impact.

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