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Preparation method of inactivated newcastle disease vaccine

A technology for chicken Newcastle disease and Newcastle disease, applied in the field of preparation of inactivated chicken Newcastle disease vaccine

Active Publication Date: 2015-05-20
山东滨州沃华生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compound traditional Chinese medicine extract is used as immune enhancer to prepare inactivated chicken Newcastle disease vaccine, which has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of inactivated chicken Newcastle disease vaccine

[0029] (a) Virus liquid inactivation:

[0030] (a1) Preparation of virus liquid: Dilute the Newcastle disease seed virus, shake well, inoculate SPF chicken embryos in the allantoic cavity of an automatic inoculation machine, inoculate 0.1ml per embryo, continue incubation in a 37°C incubator, and harvest dead embryos for 48-120 hours And 120 hours later, the allantoic fluid of live embryos was stored in a sterile container, and stored at -20°C after sampling;

[0031] (a2) Inactivation: thaw the frozen embryo fluid, inactivate after clarification and filtration, and directly inactivate the embryo fluid that has not been frozen. Mix, the final concentration of the formaldehyde solution is 0.2%, and then inactivate at 37°C for 24-26 hours (start timing when it is placed in the greenhouse), and shake 5-6 times during the period;

[0032] (a3) Blocking inactivation: add filter-sterilized 50% ...

Embodiment 2

[0040] Test items standard regulation test result Exterior Milky white slightly yellow emulsion Milky white slightly yellow emulsion dosage form water in oil type water in oil type viscosity meets the requirments Compliance stability Draw 10ml of the vaccine into the centrifuge tube, centrifuge at 3000r / min for 15 minutes, the water precipitated at the bottom of the tube should not exceed 0.5ml Anhydrous phase precipitation

[0041] A. Safety inspection

[0042] Use 6 SPF chickens aged 30-60 days, inject 1.0ml of vaccine subcutaneously in each muscle or neck and back, and observe for 14 days. There should be no local or systemic adverse reactions caused by the vaccine.

[0043] Test results: There were no local or systemic adverse reactions caused by the vaccine.

[0044] B. Efficacy test: test by serological method, if the result does not meet the requirements, it can be tested by immune challenge method.

[0045] (1) Sero...

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Abstract

The invention relates to a preparation method of an inactivated newcastle disease vaccine. The preparation method comprises the steps of virus liquid inactivation, preparation of a compound traditional Chinese medicine extract, preparation of an aqueous phase, preparation of an oil phase and emulsification. According to the preparation method, the compound traditional Chinese medicine extract serving as an immunopotentiator is added to the inactivated newcastle disease vaccine so as to well cause cellular immunologic response and enhance the immunologic function of an organism, so that an antibody is generated in advance, and the antibody titer is obviously improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological product cultivation, relates to a method for preparing an inactivated chicken Newcastle disease vaccine, in particular to a method for preparing an inactivated chicken Newcastle disease vaccine by adding a compound Chinese medicine extract as an immune enhancer to the inactivated chicken Newcastle disease vaccine . Background technique [0002] Immunopotentiators refer to substances that can be used with antigens at the same time or in advance to enhance the body's immune response to antigens or change the type of immune response. Among the existing biological, bacterial, chemical and nutritional immunopotentiators, many have good adjuvant activity, but there are still many disadvantages. Although aluminum salt adjuvants can absorb antigens and release them slowly, stimulate immune cells for a long time and enhance the body's humoral immune response, they also have many shortcomings...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/17A61P31/14
Inventor 董新荣郝圣峰张静
Owner 山东滨州沃华生物工程有限公司
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