6'-O-caffeoyl-arbutin and derivatives thereof, and application of compound prescription in preparing cosmetics or medicines
A technology for caffeoyl arbutin and cosmetics, which is applied in the field of medicine, can solve the problem of not finding melanin activity, etc., and achieve the effects of strong inhibitory effect, low acute toxicity effect, and strong inhibitory effect.
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Embodiment 1
[0029] The effect of 6'-O-caffeoyl-arbutin (6'-O-caffeoyl-arbutin) (abbreviation: KIBVA) on the inhibition of zebrafish melanin:
[0030] 1. The preparation of 6'-O-caffeoylarbutin (6'-O-caffeoyl-arbutin) (abbreviation: KIBVA): adopt the Chinese patent "patent number: ZL200710065956.X" authorized by the applicant, the method The obtained 6'-O-caffeoyl arbutin has high yield and high purity, simple and easy preparation process, short production cycle, and easy batch preparation and industrial production process. The prepared KIBVA structural formula is as follows:
[0031] .
[0032] 2. Zebrafish selection:
[0033] The 6 hpf AB line zebrafish were observed under a dissecting microscope, and the normal and consistent zebrafish were picked and transferred into 6-well microwell plates, with 30 fish per well. (Note: hpf = hour post fertilization in the present invention, Chinese refers to the hours after fertilization of zebrafish, such as 6 hpf refers to 6 hours after fertil...
Embodiment 2
[0051] Zebrafish Melanin Inhibition Recovery Assay:
[0052] 1. Zebrafish selection:
[0053] The 6 hpf AB line zebrafish were observed under a dissecting microscope, and the normal and consistent zebrafish were picked and transferred into 6-well microwell plates, with 30 fish per well.
[0054] 2. Compound treatment:
[0055] Three experimental groups were set up in the experiment, namely A, B, and C groups, wherein A group was a blank control (control) treatment group containing 3 mL of aquaculture water; B group was a solvent (0.1% DMSO, that is, 0.1% dimethyl sulfoxide) control treatment group, that is, 3 mL of aquaculture water containing 3 μL DMSO; group C is a 200 μM KIBVA treatment group, that is, a 200 μM KIBVA solution prepared with 3 mL of aquaculture water.
[0056] For each treatment group, 30 6 hpf zebrafish with the same development were selected, and they were cultured in a 28°C incubator to 52 hpf and then photographed; KIBVA was removed 24 h after the photo...
Embodiment 3
[0061] Acute toxicity test of KIBVA:
[0062] 1. Zebrafish selection:
[0063] The 6 hpf AB line zebrafish were observed under a dissecting microscope, and the normal and consistent zebrafish were picked and transferred into 6-well microwell plates, with 30 fish per well.
[0064] 2. Compound treatment:
[0065] This experiment adopts hydrostatic test, and according to the pre-experimental results, 6 different concentrations of KIBVA and arbutin (both are respectively: 0 μM, 10 μM, 100 μM, 1 mM, 2 mM, 3 mM) were set, each 30 6 hpf zebrafish with the same concentration were put into 30 tails of 6 hpf zebrafish, which were cultured in a 28°C incubator in the dark, and the mortality rate of zebrafish was used as an evaluation index.
[0066] 3. Test results:
[0067] Through the test result recording and statistical analysis, it was found that:
[0068] (1) When zebrafish were treated with drugs up to 52 hpf, it was observed that the maximum non-lethal concentration of zebraf...
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