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Preparation method and application of total extractive of artemisia rupestris L

A technology for extracts and compounds, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, and achieves the effect of simple and easy production methods.

Inactive Publication Date: 2013-06-05
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no literature records or research reports on the application of total sesquiterpenes and total flavonoids in the total extract of Artemisia annua as active ingredients in the treatment of influenza.

Method used

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  • Preparation method and application of total extractive of artemisia rupestris L
  • Preparation method and application of total extractive of artemisia rupestris L
  • Preparation method and application of total extractive of artemisia rupestris L

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of total extract of Artemisia argyi:

[0022] Grind 10kg of the dried whole Artemisia herb into 2cm, add 10 times the volume concentration of 10% ethanol, heat and reflux for extraction three times at 80°C for 2 hours, cool to room temperature, filter, combine the extracts, and concentrate under reduced pressure to No alcohol smell, get alcohol extract;

[0023] Disperse the alcohol extract with 5% ethanol to contain crude drug 0.3g / ml, centrifuge (4000 rpm), filter, and adjust the pH value of the supernatant to 5 with dilute hydrochloric acid, DM-130 macroporous adsorption resin column , first eluted with 6 times the column volume of water, then 30% ethanol with 6 times the column volume concentration, collected the ethanol eluate, concentrated under reduced pressure, and dried in vacuo to dry powder to obtain 560 g of the total extract of Artemisia argyi.

Embodiment 2

[0024] Embodiment 2: the preparation of Artemisia argyi total extract:

[0025] Grind 10 kg of dried Artemisia argyi whole herb into 2 cm, add 14 times the volume concentration of 30% ethanol, heat and reflux for extraction three times at 75°C for 1.5 hours, cool to room temperature, filter, combine extracts, and concentrate under reduced pressure to No alcohol smell, get alcohol extract;

[0026] Disperse the alcohol extract with 5% ethanol to contain crude drug 0.2g / ml, centrifuge (4000 rpm), adjust the pH value of the supernatant to 3 with dilute hydrochloric acid, AB-8 macroporous adsorption resin column, First eluted with 4 times the column volume of water, then 5 times the column volume with 40% ethanol, collected the ethanol eluate, concentrated under reduced pressure, and dried in vacuo to dry powder to obtain 600 g of the total extract of Artemisia twigs.

Embodiment 3

[0027] Embodiment 3: Preparation of the total extract of Artemisia argyi:

[0028] Grind 10kg of dried Artemisia argyi whole herb into 2cm, add 16 times the volume concentration of 50% ethanol, heat and reflux for extraction three times at 75°C for 2 hours, cool to room temperature, combine the extracts, and concentrate under reduced pressure until alcohol-free Taste, to obtain alcohol extract;

[0029] Add 10% ethanol to the extract to disperse the crude drug to 0.3g / ml, centrifuge (4000 rpm), adjust the pH of the supernatant to 6 with dilute hydrochloric acid, use HPD-450 macroporous adsorption resin column, first use Elute with 8 times the column volume of water, and then elute with 6 times the column volume of 50% ethanol, collect the ethanol eluate, concentrate under reduced pressure, and dry in vacuo to dry powder to obtain 510 g of the total extract of Artemisia twigs.

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Abstract

The invention relates to a preparation method and application of a total extractive of artemisia rupestris L. The method comprises the following steps of: crushing a dry artemisia rupestris L. medicinal material, extracting by using ethanol as an organic solvent, carrying out resin separation and purification to obtain the total extractive of the artemisia rupestris L., wherein the total extractive of the artemisia rupestris L. mainly contains sesquiterpenoids and flavonoid constitutes, a sesquiterpenoid and the flavonoid constitute compound is in the mass percentage of 25-90%, a sesquiterpenoid compound is in the mass percentage of 5-25%, a flavonoid constitute compound is in the mass percentage of 20-65%, and rupestonic acid of the sesquiterpenoid compound is in the mass percentage of 3-15%. Proved by a primary activity screening test, the total extractive of the artemisia rupestris L., obtained by using the method provided by the invention, has remarkable in vitro and vivo influenza virus resistance, bacteriostasis and sterilization effects and can also be used for effectively preventing influenza viruses. The method disclosed by the invention is simple in process, free of high-temperature and high-pressure equipment, low in production cost, stable in quality of effective components and easy to control.

Description

technical field [0001] The invention relates to a preparation method of the total extract of Artemisia chinensis and the use of the total extract in preparing medicine for treating influenza, belonging to the technical field of ethnic medicine. Background technique [0002] Influenza, referred to as influenza, is an acute respiratory infectious disease caused by influenza virus. It is highly contagious and has a high incidence rate, and it is easy to cause outbreaks or pandemics. The chemical drugs amantadine, rimantadine, zanamivir, ribavirin, etc. currently used to prevent and treat influenza all have side effects to varying degrees. And the unique antiviral curative effect of traditional Chinese medicine has obtained the general recognition of the whole world, and Chinese medicine treatment influenza is "multi-component, multi-path, multi-target" onset, and side effect is low, therefore, seeks and develops the medicine for the treatment of influenza from Chinese medicine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P11/00A61P31/16
Inventor 阿吉艾克拜尔·艾萨贺飞赵江瑜贺耀武巴杭
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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