In-vitro retina light stimulation device and method suitable for patch clamp experiment

Abstract

The invention relates to an in-vitro retina light stimulation device and a method suitable for patch clamp experiments, and belongs to the field of methods and means for neurobiological research. The invention is provided aiming at the problems that patch clamp systems in the prior art only have electrical stimulators but do not have light stimulators, and visual stimulators used for psychology or clinics in the prior art are not suitable for patch clamp experiments. The technical scheme key points are that the device comprises an illumination subsystem mainly comprising multicolor LEDs, a micro-display subsystem mainly comprising LCOS micro-displays, and a specially-designed coupling mirror set subsystem which are controlled by computer programs, and the method is a light stimulation method realized by combination of a stimulation light path with a microscope imaging light path. The purpose of the invention is to provide in-vitro retina patch clamp electrophysiological experiments with the light stimulation device and the method with miniaturization, high stability, high flexibility, easy installation, long service life, programmability, and high space-time resolution.

Description

technical field [0001] The invention relates to a device and method for the research of visual neurobiology, especially capable of providing programmable optical stimulation with high temporal and spatial resolution for isolated retinal patch clamp electrophysiological experiments, and belongs to the field of scientific instruments. Background technique [0002] Existing Patch Clamp System [0003] The patch clamp technique is an electrophysiological experimental technique capable of recording one or more ion channels of a single cell. Since its invention in the 1980s, the patch clamp technique has been gradually extended to many biological research fields such as physiology, neurobiology and pharmacology, and has greatly promoted the development of these fields. [0004] A practical patch clamp system not only includes the software and hardware of the patch clamp amplifier itself, but also includes micromanipulators, preamplifiers, analog-to-digital / digital-to-analog conve...

AI Technical Summary

Problems solved by technology

[0016] (1) The standard patch clamp system cannot provide photostimulation;

[0017] (2) The standard photostimulation system cannot be used in patch clamp experiments

[0018] (3) Due to space constraints, the standard patch clamp system and the standard visual stimulation system cannot be easily combined, and the image transmission system must be customized, and the customized system is large in size, poor in interchangeability and stability

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