Preparation method for composite-type lactobacillus microecological preparation

A technology of micro-ecological preparations and lactic acid bacteria, applied in the direction of bacteria, etc., can solve the problems of high cost of fermentation tanks, high cost of use, unstable products, etc., and achieve the effect of long storage time, powerful functions, and improved water quality indicators

Inactive Publication Date: 2013-06-12
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, most of the products coated with lactic acid bacteria probiotics are expensive, and the cost of use is very high; while the products of lactic acid bacteria probiotics are relatively cheap, but most of the products are very unstable and the number of viable bacteria is low. The storage period is very short, and the transportation cost is high; currently, most of the solid and liquid lactic acid bacteria probiotics are fermented by fermentation tanks. On the one hand, the production process is strict and requires professional technicians to operate; higher cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of a compound lactic acid bacteria micro-ecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the The lactic acid bacteria strains were purified and selected to produce three lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then the lactic acid bacteria strains selected after purification and breeding were expanded and cultivated to prepare composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:

[0027] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;

[0028] (2) Purify and select three lactic acid...

Embodiment 2

[0039] A preparation method of a compound lactic acid bacteria micro-ecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the Purify and select four lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then expand the culture of the selected lactic acid bacteria strains after purification and breeding to prepare a composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:

[0040] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;

[0041] (2) Purify and select four lactic acid bacteria strains with large calcium-dissolving circle, fast...

Embodiment 3

[0052] A preparation method of a compound lactic acid bacteria micro-ecological preparation, using cultured animals, cultured water and bottom mud as bacteria sources, isolating lactic acid bacteria strains by coating solid MRS medium after enriching in MRS liquid medium; and then separating the The lactic acid bacteria strains were purified and selected to produce five lactic acid bacteria strains with large calcium-dissolving circles, fast growth and strong vitality; then the lactic acid bacteria strains selected after purification and breeding were expanded and cultivated to prepare composite seed liquid; finally, the composite seed liquid Composite lactic acid bacteria probiotics can be obtained by inoculation and fermentation:

[0053] (1) With cultured animals, cultured water and sediment as the source of bacteria, lactic acid bacteria strains are isolated by coating solid MRS medium after enrichment in MRS liquid medium;

[0054] (2) The isolated lactic acid bacteria st...

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PUM

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Abstract

The invention discloses a preparation method for a composite-type lactobacillus microecological preparation, and belongs to the field of lactobacillus fementation. The preparation method comprises the following steps of: separating lactobacillus strains by taking bred animals, culture water body and bottom sediment as bacterial sources; then purifying the separated lactobacillus strains to selectively breed 2-5 lactobacillus strains with large calcium-dissolving rings, fast growth speed and strong activity; then carrying out enlarging culture on the lactobacillus strains which are purified and selectively bred, and preparing composite seed solution; and finally, inoculating and fermenting the composite seed solution to obtain the composite-type lactobacillus microecological preparation. Compared with the prior art, the preparation method disclosed by the invention has the beneficial effects of simple culture equipment and operation, and stable storage time compared with a single strain fermentation water agent.

Description

technical field [0001] The invention relates to the field of lactic acid bacteria fermentation, in particular to a preparation method of a compound lactic acid bacteria microecological preparation. Background technique [0002] With the rapid development of the aquaculture industry, lactic acid bacteria preparations have attracted more and more attention because of their natural, non-toxic, no side effects, no pollution, no residue, safe and reliable advantages. As a beneficial "bioremediation agent" for purifying water, the application of lactic acid bacteria preparations in aquaculture industry has attracted more and more attention. Splash on bodies of water. Among them, the research on mixing and feeding and fermented feed focuses on the inhabitation of lactic acid bacteria in the intestines and stomach of aquatic animals, and the regulation of animal intestinal flora structure and changes in feed structure. Sprinkling to regulate water quality is closely related to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 薛爱琴罗国强苏艳秋顾继锐
Owner TONGWEI
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