Artificial hybridization method for improving peony breeding fecundity

Abstract

The invention relates to an artificial hybridization method for improving peony breeding fecundity. The artificial hybridization method comprises the steps of: (1). collecting male parent pollen and carrying out refrigerated storage of the male parent pollen; (2). cutting off a part of a female parent petal, removing anther, dipping pollen germination fluid, smearing the pollen germination fluid on a female parent stigma, dipping pollen, smearing the pollen on the surface of the stigma, and then bagging a hybrid flower for isolation; (3). hanging a tag on a corresponding branch of a hybrid plant, and establishing a hybrid file; and (4). after a peony flowering phase, punching a through hole in the isolating bag, retaining the isolating bag until a seed develops to a certain degree, and harvesting timely. The peony artificial hybridization method provided by the invention effectively guarantees the pollen vitality through low-temperature storage of the pollen, thus solving the problem of flowering asynchronism of hybridized combination; the pollen germination fluid is manually smeared to create a pollen germination environment so as to improve the germination rate of the male parent pollen, and effectively overcome the obstacle before hybridized fertilization; and the punched isolating bag is retained, so that the functions of ventilation and rain prevention are achieved, simultaneously the intensity of sunlight direct radiation is reduced, the insect and bird bites are reduced, and the phenomena of early cracking and withering of capsules are reduced.

Description

technical field [0001] The invention relates to the field of plant hybridization and breeding, in particular to an artificial hybridization method for improving the fruitiness of peony breeding. Background technique [0002] At present, peony hybridization is mainly carried out between peony varieties that meet in the natural flowering period. Fresh pollen is collected for hybridization between parental materials. Remove, routine management. In hybridization, pollen vigor is not guaranteed, there are no effective protection measures in the growth process, poor firmness, and small seed collection. Store pollen at room temperature. During the hybridization process, when the flowering period of the parental material does not meet, the pollen activity cannot be guaranteed; during pollination, because the female stigma may not have the environmental conditions for pollen germination, the pollen cannot germinate and die, and thus cannot be fertilized, affecting fruiting; isolatio...

AI Technical Summary

Problems solved by technology

In hybridization, pollen vigor is not guaranteed, there are no effective protection measures in the growth process, poor firmness, and small seed collection

Store pollen at room temperature. During the hybridization process, when the flowering period of the parental material does not meet, the pollen activity cannot be guaranteed; when pollinating, because the female parent's stigma may not have the environmental conditions for pollen germination, the pollen cannot germinate or die, and thus cannot be fertilized, affecting fruiting; isolation bag If it is removed in time after the flowering period, due to direct sunlight, wind and rain, insect bites and other reasons, a large number of peony pods will not mature normally and will crack, die or be damaged in advance

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