Method for determining content of amylopectin and amylose in broomcorn

A technology of amylose content and amylopectin, which can be used in the measurement of color/spectral characteristics, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., which can solve the problems that are easy to cause errors , reduce the detection accuracy, cumbersome detection methods and other problems, to achieve the effect of high accuracy and simple calculation method

Inactive Publication Date: 2013-06-19
CHONGQING UNIV
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Problems solved by technology

Among them, the method for simultaneous detection of two components is the most widely used dual-wavelength method. This method has been used for the determination of starch content in wheat, chestnut, corn, cassava, and potato. However, this method involves four wavelengths, including two The measurement wavelength and the two reference wavelengths determined by drawing, so the detection method is cumbersome, and the assumption of dual wavelengths is likely to cause errors, thereby reducing the detection accuracy

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  • Method for determining content of amylopectin and amylose in broomcorn
  • Method for determining content of amylopectin and amylose in broomcorn
  • Method for determining content of amylopectin and amylose in broomcorn

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Embodiment

[0046] Embodiment: a kind of assay method of amylopectin and amylose content in sorghum, comprises the steps:

[0047] S1: Prepare amylopectin regression equation and amylose regression equation:

[0048] S11: Weigh 100.0 mg of pure amylopectin and 100.0 mg of pure amylose and place them in 100 mL beakers respectively,

[0049] Add 1ml of ethanol with a concentration of 95% to the two beakers for dispersion treatment, add 9mL of 1mol / L sodium hydroxide solution after treatment and mix well, seal the mouth of the beaker with plastic wrap, stir in a boiling water bath for 10min, and wait until After the pure amylose and amylopectin are completely dissolved, transfer them to 100mL volumetric flasks and dilute to the mark with distilled water to obtain amylose standard solution and amylopectin standard solution with a concentration of 1.0mg / mL.

[0050] S12: Preparation of amylopectin regression equation: Pipette 4mL, 5mL, 6mL, 7mL, 8mL, 9mL of the standard solution of amylopecti...

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Abstract

The invention relates to a method for determining content of amylopectin and amylose in broomcorn. The method comprises the following steps of 1, preparing an amylopectin regression equation and an amylose regression equation; 2, calculating the absorption ratio alpha of the amylopectin and the absorption ratio beta of the amylose; 3, measuring the sample, calculating the absorbance of the amylopectin and the amylose at the measurement wavelength through a given formula, and then correspondingly inserting the absorption ratio and the absorbance into corresponding regression equations to obtain the concentration of the amylopectin and the amylose. The determination method provided by the invention just requires two wavelengths; an error caused by dual-wavelength null hypothesis is removed; and compared with the dual-wavelength, the method disclosed by the invention is simpler in principle and computing method, and the finally obtained content of the amylopectin and the amylose is high in accuracy.

Description

technical field [0001] The invention relates to a method for detecting starch content in plants, in particular to a method for measuring amylopectin and amylose content in sorghum. Background technique [0002] Sorghum has been planted in China for thousands of years, and it is widely distributed in China. China also has a long history of liquor brewing. Sorghum is one of the main raw materials for brewing, and its starch content and the ratio of amylose to amylopectin have an important relationship with the quality and yield of wine making. Varieties with higher amylopectin content, During the fermentation process, it is easy to meet the technical standards of soft ripening, clearing, sweating, etc., and the utilization rate of starch is high, so it is regarded as a high-quality raw material for wine making. Therefore, it is necessary to explore a fast and simple method to determine the content of the two components during raw material procurement and crop breeding. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33
Inventor 霍丹群蒋兰侯长军王洪彬
Owner CHONGQING UNIV
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