Composite of Antrodia camphorata entity and shell-broken ganoderma lucidum spore powder and application thereof in immune adjustment
A technology of broken ganoderma lucidum spore powder and composition, applied in the field of food and medicine
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Embodiment 1
[0030] Preparation of Antrodia camphorata fruiting body powder:
[0031] Select Ф20-40cm camphor wood and cut it into basswood with a length of about 20-30cm. After the basswood is bagged, it is sterilized in a sterilizing cabinet at a temperature of 120-122°C for 14 hours.
[0032] In the culture room, aseptically inoculate the parent species of Antrodia camphorata into the original seed culture bottle, the culture medium is agar medium, and culture at a temperature of 25-29°C and a humidity of 75%-85% for 7-7 10 days. Then, the original seed is aseptically inoculated into the cultivar culture bottle, and the culture medium is the agar medium, and the temperature is 25-29°C and the humidity is 75%-85%, and the culture is cultivated for 7-10 days to obtain the cultivar; A kind of sterile inoculation in cinnamomum camphora basswood. The inoculation site is the surface of basswood. The inoculum is planted on the basswood with 200-300 bacterial balls of 3-5 mm in size.
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Embodiment 2
[0037] Preparation of Antrodia camphorata fruit body and broken Ganoderma lucidum spore powder composition:
[0038] 2.1 Materials
[0039] The Antrodia camphorata fruit body powder prepared according to the method provided in Example 1; the broken Ganoderma lucidum spore powder is a commercially available broken spore powder.
[0040] 2.2 Method
[0041] 2.2.1 Preparation of the composition
[0042] Accurately weigh three parts of the broken Ganoderma lucidum spore powder, whose masses are 1.00g, 2.00g and 4.00g respectively; Three parts of the broken ganoderma spore powder and the other three parts of the fruiting body powder of Antrodia camphorata are mixed evenly to obtain three compositions of the broken ganoderma spore powder and the fruiting body of Antrodia camphorata. In terms of mass ratio, the proportions of broken ganoderma lucidum spore powder and Antrodia camphorata fruiting body powder are: 1:1, 2:1, 4:1 respectively, which are denoted as LZ-1, LZ-2, and LZ-4...
Embodiment 3
[0058] Immunomodulatory activity experiments and results:
[0059] 3.1 Materials
[0060] 3.1.1 Test drugs:
[0061] Single formula of broken Ganoderma lucidum spore powder (hereinafter referred to as "Ganoderma lucidum"), brown powder, stored at room temperature; Single formula of Antrodia camphorata fruiting body powder (hereinafter referred to as "Antrodia camphorata"), khaki powder, stored at room temperature; Broken Ganoderma lucidum spore powder / Antrodia camphorata fruiting body powder 1:1 formula (hereinafter referred to as "Lingzhang 1:1"), brown powder, stored at room temperature; broken wall Ganoderma lucidum spore powder / Antrodia camphorata fruiting body powder 2:1 formula (hereinafter referred to as "Lingzhang 2: 1"), brown powder, stored at room temperature; 4:1 formula of broken Ganoderma lucidum spore powder / Antrodia camphorata fruiting body powder (hereinafter referred to as "Lingzhang 4:1"), brown yellow powder, stored at room temperature; positive control d...
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