Solid sample carrying stage, spectrophotometer and LSPR (localized surface plasma resonance) detection method

Abstract

The invention provides a solid sample carrying stage for a spectrophotometer. The solid sample carrying stage comprises an adjusting seat and a moving frame, wherein the adjusting seat is L-shaped, and comprises a base part and an arm part, the base part is provided with a groove, a first guide rail groove is formed in the base part, and a screw hole and an ejector pin through hole are formed in the arm part; the moving frame is U-shaped, comprises a base connecting part, a first arm part and a second arm part; the base connecting part is embedded in the groove of the base part of the adjusting seat, a second guide rail groove is formed in the base connecting part, the first guide rail groove is matched with the second guide rail groove to form a complete guide rail groove and a guide rail is embedded in the complete guide rail groove, clamping grooves are formed in the upper sides of the first arm part and the base connecting part, and a through hole and an ejector pin through hole are formed in the second arm part; and a moving knob is in threaded connection with the screw hole of the arm part and penetrates through the screw hole and the through hole of the second arm part, and the ejector pin penetrates through the ejector pin through hole of the arm part and the ejector pin through hole of the second arm part. The invention further provides a corresponding LSPR detection method. The solid sample carrying stage and the LSPR detection method provided by the invention are very convenient for high-flux detection and suitable for measuring a sample with a small width.

Description

technical field [0001] The invention relates to the technical fields of optical platform, analytical chemistry, etc., in particular, the invention relates to a solid sample stage, a spectrophotometer and an LSPR detection method. Background technique [0002] A spectrophotometer is an instrument for qualitative and quantitative analysis of the substance by measuring the light absorbance of the substance under test at a specific wavelength or within a certain wavelength range. At present, spectrophotometer has been widely used in analytical chemistry, molecular biology and other fields. It usually includes light source, monochromator, sample chamber, detector, signal processor, storage system and display system. The state of the object measured by the spectrophotometer mainly includes two categories: liquid and solid. [0003] Among them, the liquid substance is usually placed in the cuvette and evenly distributed in the cuvette, so generally only one cuvette is placed in o...

AI Technical Summary

Problems solved by technology

First, once the solid sample is fixed, it cannot move flexibly during the measurement process, resulting in only one fixed area of ​​the sample being measured at a time, making it difficult to perform high-throughput detection of the sample using a spectrophotometer; second, The width of the solid sample cannot be smaller than the gap between the two elastic metal sheets, otherwise the solid sample will not be firmly clamped by the metal sheets

However, the width of biochips is generally small, so the above-mentioned supports are difficult to be used for the determination of biochips.

Images