Combined detection method for multiple vitamin D metabolites and detection kit and application thereof
A technology for metabolites and vitamins, applied in the field of analysis and detection, to achieve the effects of saving sample pretreatment time, high sensitivity, and avoiding interference
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] This embodiment provides a method for detecting vitamin D metabolites, which is detected by liquid chromatography tandem mass spectrometry, specifically as follows:
[0046] 1. Sample pretreatment
[0047] Add 200 μL of serum samples, standards or quality control products in sequence to a 5 mL glass tube, then add 50 μL of mixed internal standard solution and 200 μL of methanol in sequence, and mix thoroughly; then add 500 μL of 0.1M zinc sulfate aqueous solution, 1 mL of n-hexane and ethyl acetate (The volume ratio of n-hexane and ethyl acetate is 8:2), vortex, mix well, centrifuge at 12000g for 5min, transfer 750μL of the supernatant to a 2mL 96-well plate, and dry it with nitrogen (37℃) ; Add 100 μL methanol aqueous solution to redissolve (V:V=5:5) to obtain the sample to be tested. Take 40 μL of the sample to be tested for LC-MS / MS analysis and detection.
[0048] Mixed internal standard solution: the concentration of each metabolite is: 25OHD 3 -d 3 (50ng / mL), ...
Embodiment 2
[0070] This embodiment provides a method for detecting vitamin D metabolites. The method is detected by liquid chromatography tandem mass spectrometry. The difference from Example 1 is that in mobile phase A and mobile phase B, the concentration of lithium acetate is 0.22mM.
experiment example 1
[0071] Experimental example 1 Detection of separation effect of vitamin D metabolites
[0072] The detection method of Example 1 is used to carry out liquid phase separation of vitamin D metabolites and their interfering structural analogs, and the results are as follows figure 1 and figure 2 As shown, the results show that the detection method of the present invention can well 25-hydroxyvitamin D3, 25-hydroxyvitamin D2, 1,25 dihydroxyvitamin D3, 1,25 dihydroxyvitamin D2, 24,25 dihydroxyvitamin Six vitamin metabolites, D3, 24,25 dihydroxyvitamin D2, were separated and separated from the interfering substance 3-epi 25(OH)D 3 , 3-epi 24,25(OH) 2 D. 3 、23R,25(OH)D 3 , 4β,25(OH) 2 D is well separated.
PUM
Property | Measurement | Unit |
---|---|---|
recovery rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com