Cell line for detecting I type interferon biological activity and application thereof
A biological activity and interferon technology, which is applied in the field of detecting the biological activity of animal type I interferon, can solve the problems of affecting versatility, narrow application range, low sensitivity and accuracy, etc., and achieves convenient high-throughput detection. , the effect of saving testing time
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[0022] Interferon is an important antiviral substance, and the quantitative detection of its biological activity has important basic research and production application significance. The Mx promoter is one of the main effector transcriptional promoters of interferon, which expresses Mx protein under the action of type I IFN.
[0023] In one embodiment, the present invention clones the chicken Mx promoter (Mxp), the sequence of which is different from the reported ones, with a difference of 6 bases. A luciferase (luciferase, luc) reporter gene was connected downstream to construct the chicken type I interferon activity detection plasmid pMxp-luc. The DF-1 chicken embryo fibroblast cell line was transiently transfected with pMxp-luc, and the cells were treated with chicken IFN-α / β 24 hours later. As a result, the luciferase gene was transcribed and expressed under the action of the Mx promoter; luciferase The expression level of interferon has a good linear correlation with the...
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