A method for enriching Monascus by using retrograded starch

Abstract

The invention discloses a method for gathering monascus by retrograded starch, and provides a method capable of reducing reproduction of saccharomycetes and bacteria to permit monascus spores to germinate and grow. The method comprises the steps of pasting starch emulsion for 30 minutes at 90-98 DEG C, carrying out high-pressure treatment to obtain starch paste; aging for 96-120 hours at 0-4 DEG C to obtain colloidal retrograded starch; putting the colloidal retrograded starch in air of which the temperature is -30 to -5 DEG C to naturally ventilate or manually blast, wherein the air speed is 5-15m / s; freezing the retrograded starch; slowly gasifying the moisture, wherein the dried starch generates a gap; the monascus spores in the air enter the dried starch gap under drive of wind; and the monascus spores are gathered and planted in the process of drying the retrograded starch. By adopting the method, reproduction of a large part of mycetes, saccharomycetes and bacteria are restrained in a low-temperature environment; the retrograded starch is frozen and gasified and forms a foam structure; and a condition is created for reproduction of aerobic monascus.

Description

technical field

[0001] The invention relates to the field of food biotechnology, in particular to a method for enriching monascus by utilizing retrograded starch. Background technique

[0002] Monascus sp. is often found in dairy products, koji workshops and Daqu, etc., and it is usually cultured on wort agar in the laboratory. The enrichment of Monascus in nature is often done by adding alcohol, lactic acid, streptomycin, etc. When Monascus is collected, a large number of other acid-resistant, alcohol-resistant and streptomycin-resistant molds, yeasts and bacteria will also be enriched, which will bring difficulties to the subsequent strain separation and increase the workload of strain isolation. Contents of the invention

[0003] The object of the present invention is to aim at the technical defects existing in the prior art, and provide a method for enriching Monascus by utilizing retrograded starch, which can reduce the reproduction of yeast and bacteria and allow Mo...

Examples

Embodiment 1

[0019] Corn starch was dissolved in water to obtain a starch emulsion with a concentration of 10% by mass, and the starch emulsion was gelatinized at 90°C for 30 minutes, followed by high-pressure treatment to obtain starch paste. The temperature of the high pressure treatment is 120° C., and the time of the high pressure treatment is 60 minutes. Afterwards, it was aged at 4°C for 96 hours to obtain gelatinous retrograded starch. Put the colloidal retrograded starch in the air with an outdoor temperature of -15°C and a wind speed of 5-15 m / s. The retrograded starch will freeze, and the water in it will slowly gasify. Monascus spores in the air will be blown into the starch, and the starch will enrichment and growth. The result of enrichment is as figure 1 shown.

[0020] Grind the above solid red starch with Monascus into powder and put it into sterile water to make a bacterial suspension, and apply the bacterial suspension to the mold solid medium (5% soluble starch, 4% ma...

Embodiment 2

[0022] Dissolve sweet potato starch in water to obtain a starch emulsion with a mass percentage of 15%, gelatinize the starch emulsion at 90°C for 30 minutes, and then perform high-pressure treatment to obtain starch paste. The temperature of the high-pressure treatment is 120°C, and the time of high-pressure treatment is 60 minutes. . Afterwards, it was aged at 4°C for 96 hours to obtain gelatinous retrograded starch. Put the colloidal retrograded starch in the air with an indoor temperature of -28°C, artificially blow the wind at a wind speed of 5-15 m / s, the retrograded starch will freeze, the water in it will slowly vaporize, and the Monascus spores in the air will be blown into In starch, enrich and grow in starch.

[0023] Grind the solid red starch with Monascus into powder and put it into sterile water to make a bacterial suspension, and apply the bacterial suspension to the mold solid medium (5% soluble starch, 4% maltose, 3% peptone, agar 2%, others are water), cul...

Embodiment 3

[0025] Dissolve potato starch in water to obtain a starch emulsion with a mass percentage of 13%, gelatinize the starch emulsion at 90°C for 30 minutes, then perform high-pressure treatment to obtain starch paste, the temperature of the high-pressure treatment is 120°C, and the time of high-pressure treatment is 60 minutes ; After that, aged at 4°C for 96h to obtain colloidal retrograded starch. Put the colloidal retrograded starch outdoors, artificially blow the air, the air temperature is -15°C, the wind speed is 5-15 m / s, the retrograded starch is frozen, the water in it is slowly vaporized, and the Monascus spores in the air are blown into the starch , enrich and grow in starch.

[0026] Grind the solid red starch with Monascus into powder and put it into sterile water to make a bacterial suspension, and apply the bacterial suspension to the mold solid medium (5% soluble starch, 4% maltose, 3% peptone, agar 2%, others are water), cultivated at 32°C for 5-7 days, a single ...

technical field

[0001] The invention relates to the field of food biotechnology, in particular to a method for enriching monascus by utilizing retrograded starch. Background technique

[0002] Monascus sp. is often found in dairy products, koji workshops and Daqu, etc., and it is usually cultured on wort agar in the laboratory. The enrichment of Monascus in nature is often done by adding alcohol, lactic acid, streptomycin, etc. When Monascus is collected, a large number of other acid-resistant, alcohol-resistant and streptomycin-resistant molds, yeasts and bacteria will also be enriched, which will bring difficulties to the subsequent strain separation and increase the workload of strain isolation. Contents of the invention

[0003] The object of the present invention is to aim at the technical defects existing in the prior art, and provide a method for enriching Monascus by utilizing retrograded starch, which can reduce the reproduction of yeast and bacteria and allow Mo...

Examples

Embodiment 1

[0019] Corn starch was dissolved in water to obtain a starch emulsion with a concentration of 10% by mass, and the starch emulsion was gelatinized at 90°C for 30 minutes, followed by high-pressure treatment to obtain starch paste. The temperature of the high pressure treatment is 120° C., and the time of the high pressure treatment is 60 minutes. Afterwards, it was aged at 4°C for 96 hours to obtain gelatinous retrograded starch. Put the colloidal retrograded starch in the air with an outdoor temperature of -15°C and a wind speed of 5-15 m / s. The retrograded starch will freeze, and the water in it will slowly gasify. Monascus spores in the air will be blown into the starch, and the starch will enrichment and growth. The result of enrichment is as figure 1 shown.

[0020] Grind the above solid red starch with Monascus into powder and put it into sterile water to make a bacterial suspension, and apply the bacterial suspension to the mold solid medium (5% soluble starch, 4% ma...

Embodiment 2

[0022] Dissolve sweet potato starch in water to obtain a starch emulsion with a mass percentage of 15%, gelatinize the starch emulsion at 90°C for 30 minutes, and then perform high-pressure treatment to obtain starch paste. The temperature of the high-pressure treatment is 120°C, and the time of high-pressure treatment is 60 minutes. . Afterwards, it was aged at 4°C for 96 hours to obtain gelatinous retrograded starch. Put the colloidal retrograded starch in the air with an indoor temperature of -28°C, artificially blow the wind at a wind speed of 5-15 m / s, the retrograded starch will freeze, the water in it will slowly vaporize, and the Monascus spores in the air will be blown into In starch, enrich and grow in starch.

[0023] Grind the solid red starch with Monascus into powder and put it into sterile water to make a bacterial suspension, and apply the bacterial suspension to the mold solid medium (5% soluble starch, 4% maltose, 3% peptone, agar 2%, others are water), cul...

Embodiment 3

[0025] Dissolve potato starch in water to obtain a starch emulsion with a mass percentage of 13%, gelatinize the starch emulsion at 90°C for 30 minutes, then perform high-pressure treatment to obtain starch paste, the temperature of the high-pressure treatment is 120°C, and the time of high-pressure treatment is 60 minutes ; After that, aged at 4°C for 96h to obtain colloidal retrograded starch. Put the colloidal retrograded starch outdoors, artificially blow the air, the air temperature is -15°C, the wind speed is 5-15 m / s, the retrograded starch is frozen, the water in it is slowly vaporized, and the Monascus spores in the air are blown into the starch , enrich and grow in starch.

[0026] Grind the solid red starch with Monascus into powder and put it into sterile water to make a bacterial suspension, and apply the bacterial suspension to the mold solid medium (5% soluble starch, 4% maltose, 3% peptone, agar 2%, others are water), cultivated at 32°C for 5-7 days, a single ...

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