High-specific and high-sensitive detection method for tuberculosis flora

Abstract

The invention discloses a high-specific and high-sensitive detection method for tuberculosis flora. The high-specific and high-sensitive detection method includes 1), preparing reaction liquid, 2), placing the reaction liquid in a baking oven at the temperature of 63 DEG C to perform reaction for 30-60 minutes, and determining to be positive if the reaction liquid is turbid, 3), using clinical-isolated tuberculosis bacterial strain nucleic acid, clinical-isolated non-tuberculous mycobacteria nucleic acid and a plurality of nucleic acid of clinical-isolated staphylococcus capitis, staphylococcus hominis, staphylococcus epidermidis, streptococcus viridans, escherichia coli and Klebsiella to perform reaction repeatedly, and observing positive and negative natures of the nucleic acid. Through preparation of reaction liquid and design of primer sequences, the tuberculosis flora can be quickly and accurately detected and corresponding measures can be taken timely, so that incidence rate of diseases is reduced.

Description

technical field [0001] The invention relates to the technical field of disease detection, in particular to a method for detecting tuberculosis with high specificity and high sensitivity. Background technique [0002] Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis that seriously endangers human health. The number of cases worldwide exceeds 10 million each year. my country is one of the hardest-hit countries with tuberculosis. New cases reach one million people every year, seriously endangering national health, and Mycobacterium tuberculosis grows slowly. , the culture time is as long as 4 to 8 weeks, but nucleic acid detection technologies such as PCR and gene chips require expensive equipment and skilled operation, and are not easy to be popularized in primary medical institutions. The present invention provides cheap, simple and highly specific detection of human tuberculosis infection The primer sequences and methods of the flora are needed to im...