Application of pentagalloylglucose in medicines used for killing fish in-vitro ciliates

A technology for pentagalloylglucose and ciliates, which is applied in the field of killing parasite drugs, can solve the problems of polluted environment, easy residues of chemical drugs, endangering human health, etc., and achieves simple preparation process, prevention and control of cucurbita disease, and wide sources. Effect

Active Publication Date: 2014-07-02
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because these chemicals are easy to residue, pollute the environment and endanger human health, most of them have been banned in food fish.

Method used

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  • Application of pentagalloylglucose in medicines used for killing fish in-vitro ciliates
  • Application of pentagalloylglucose in medicines used for killing fish in-vitro ciliates
  • Application of pentagalloylglucose in medicines used for killing fish in-vitro ciliates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: The effect of PGG drug on the infective larvae of the multi-seed melon worm

[0022] (1) Preparation of PGG drug: Weigh 0.1g of PGG powder with an electronic balance, dissolve it in 1mL of ultrapure water, and stir evenly to obtain a grade 1 stock solution with a concentration of 100mg / mL. Take 20μL from the grade 1 stock solution and add 9.98mL of ultrapure water, stirred evenly to obtain a 2nd grade stock solution with a concentration of 200mg / L, from the 2nd grade stock solution, 1600 μL was added to 6400 μL of ultrapure water and mixed to obtain a PGG drug with a PGG concentration of 40mg / L .

[0023] (2) Add 400 μL of the PGG drug prepared in step (1) to each well in the first row of wells of the 96-well plate, take 200 μL from the first row of wells, add to the second row of wells, then add 200 μL of ultrapure water and mix well, To obtain a drug solution with a concentration of 20 mg / L, take 200 μL from the second row of holes and add it to the thi...

Embodiment 2

[0025] Embodiment 2: The killing effect of PGG drug on the cyst of the multi-seed melon worm

[0026] (1) The preparation method of the PGG medicine is the same as that in Example 1, and a PGG medicine solution with a concentration of 80 mg / L is finally obtained.

[0027] (2) Add 300 μL of the PGG medicinal solution prepared in step (1) to each well of a 24-well plate by a two-fold gradient dilution method, and add 300 μL of water containing 200 polyzygocyst cysts to each well of the 24-well plate. Add to each well so that the final concentration of the drug solution is 0 (control), 10, 20, 40 and 80 mg / L, respectively, and each group is set with 5 parallels. Record the mortality rate of the cyst at 1, 2, 3 and 4 hours under a 4x microscope, and calculate the EC 50 (See Table 2). After 4 hours, the 24-well plate was placed in an incubator, and the temperature was controlled at 23°C. After 18 hours of incubation, the average number of hatched larvae per cyst was counted.

[...

Embodiment 3

[0029] Embodiment 3: the influence of low concentration PGG drug on the infectivity of the larvae

[0030] (1) The configuration of the PGG drug was the same as that in Example 1, and finally a PGG drug solution with a concentration of 100 mg / L was obtained.

[0031] (2) In 18 transparent glass jars with a volume of 35L, 5L of aerated culture water is respectively loaded, and each liter of water contains 40,000 infected larvae, and each jar has a total of 200,000 infectious larvae, which are prepared by taking step (1) respectively. Add 25, 50, 100, 250 and 500 μL of PGG drug solution into the above-mentioned different cylinders, mix well, and control without adding medicine, the final concentration of medicine in each cylinder is 0 (control), 0.5, 1, 2, 5 and 10 mg respectively / L, each concentration was repeated 3 times, and the larvae of the infective polyzygotic melon worm were treated with the drug for 1 h in the above drug concentration. Then put 10 channel catfish (ave...

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Abstract

The invention belongs to the technical field of parasite killing medicines, and discloses an application of pentagalloylglucose in medicines used for killing fish in-vitro ciliates and especially ichthyophthirius multifilis. Within an effective dose range, 100% killing can be realized. The pentagalloylglucose has the advantages of no residue, no pollution, and no harm to human bodies. Pentagalloylglucose has great potential to be developed into novel environment-friendly medicines for controlling fish in-vitro ciliates, and has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of parasite-killing drugs, and in particular relates to the application of pentagalloyl glucose in killing fish ectoparasitic ciliates. Background technique [0002] Parasitic ciliate disease is a kind of important disease in aquaculture, and small melon worm disease is a kind of disease caused by multi-seed small melon worm in parasitic ciliate, also known as white spot disease, is a common disease in aquaculture production , frequently-occurring disease, can cause serious economic losses. For a long time, the prevention and treatment of multi-seed melon worm disease has been dominated by chemical drugs, such as malachite green, mercury preparations (mercurous nitrate and mercurous acetate) and formaldehyde. However, because these chemicals are easy to remain, pollute the environment and endanger human health, most of them have been banned in edible fish. For example, malachite green and mercury preparati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7032A61P33/14
Inventor 张其中付耀武
Owner JINAN UNIVERSITY
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