Oocyte in-vitro maturity nutrient solution and preparation method thereof

A technology for in vitro maturation culture and oocyte, which is applied to the field of in vitro maturation medium of oocytes and its preparation, which can solve the problems of inability to industrialize, unstable clinical results, unclear refinement components, etc., and achieve stable quality and commercial products. The composition is stable and safe, and the composition is stable.

Active Publication Date: 2013-09-04
JIANGSU HAOKANG BIOLOGICAL MEDICAL SCI & TECH CO LTD
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  • Abstract
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Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of oocyte maturation medium and its preparation method in vitro with stable composition and stable quality, which can be ...

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  • Oocyte in-vitro maturity nutrient solution and preparation method thereof
  • Oocyte in-vitro maturity nutrient solution and preparation method thereof
  • Oocyte in-vitro maturity nutrient solution and preparation method thereof

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Embodiment Construction

[0013] 1. Preparation of oocyte maturation medium in vitro

[0014] The preparation method of oocyte maturation medium in vitro of the present invention:

[0015] Add 0.075-0.10IU / ml FSH and 0.5-0.8IU / ml HCG to the basic cell culture medium TCM199, filter through a 0.22μm filter, store at 4°C and use within 3 weeks. Before use, add 0.1-0.2 μg / ml 17β-estradiol, 10%-20% human commercial substitute serum or human albumin, and mix thoroughly. Before culturing the oocytes, the culture medium was equilibrated in a 37°C incubator under 5% carbon dioxide concentration for more than 12 hours.

[0016] The addition and configuration are required to be carried out in a GMP workshop environment, requiring sterility, non-toxic, and dust-free.

[0017] 2. Acute toxicity and long-term toxicity test of oocyte maturation medium in vitro

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Abstract

The invention belongs to the technical field of assisted reproduction, and particularly relates to a oocyte in-vitro maturity nutrient solution and a preparation method thereof. The oocyte in vitro manturation culture medium is based on commercialized tissue nutrient solution TCM199, and accurately added commercialized constituents comprise 0.075-0.10 IU/ml FSH and 0.5-0.8 IU/ml HCG, 0.1-0.2 microgram/ml 17 beta-estradiol, and human commercialized replaced serum or human albumin with the volume percent being of 10-20 percent. The oocyte in-vitro maturity nutrient solution is stable in constituents and quality, can be industrially produced. By adding commercialized human replaced serum and serum albumin, improving the concentration of the estradiol, FSH and HCG in the culture medium and simulating in-vivo mature environment in human oocyte, a good clinical effect is obtained, the in vitro maturity rate of oocyte reaches 72.08 percent, and the clinical pregnancy rate reaches 23.71 percent.

Description

technical field [0001] The invention belongs to the technical field of assisted reproduction, and in particular relates to an oocyte maturation culture solution in vitro and a preparation method thereof. Background technique [0002] Fetal calf serum or human mature follicular fluid are mostly added to the existing culture medium for cultivating female germ cells for rapid maturation in vitro, which can easily lead to the spread of heterogeneous pathogens and the spread of unexplained diseases among the same species, and easily lead to neonatal morbidity. Defects are not conducive to prenatal and postnatal care. In addition, the detailed components of various additives are not clear, resulting in unstable clinical results and failure to industrialize. Contents of the invention [0003] The purpose of the present invention is to provide a kind of oocyte maturation medium in vitro and its preparation method with stable composition and stable quality, which can be industrial...

Claims

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Application Information

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IPC IPC(8): C12N5/075
Inventor 钱云陈娟
Owner JIANGSU HAOKANG BIOLOGICAL MEDICAL SCI & TECH CO LTD
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