High performance liquid chromatography method for determination of benzalkonium chloride content in antiseptic wipes

A high-performance liquid chromatography, benzalkonium chloride technology, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to realize detection, less disinfectant in disinfectant wipes, etc., and achieves high accuracy, simple and fast method. , the effect of eliminating the interference of compound ingredients

Inactive Publication Date: 2013-09-25
PROD QUALITY SUPERVISING & INSPECTING INST OF TAIZHOU CITY
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Problems solved by technology

Domestically reported methods for detecting DDBAC content in disinfectant include titration [1], ultraviolet spectrophotometry [2] and ion chromatography [3], but the amount of disinfectant in disinfectant wipes is small, and the detection of the above methods cannot be realized

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  • High performance liquid chromatography method for determination of benzalkonium chloride content in antiseptic wipes

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[0009] exist figure 1 Among them, the present invention provides a method for measuring the content of benzalkonium chloride in disinfectant wet wipes by high performance liquid chromatography, which comprises the following steps: step 1, weighing about 4.0 g of the disinfectant wet wipe samples, and placing them in an Erlenmeyer flask; Step 2, using acetonitrile-70 mmol·L-1 ammonium acetate and glacial acetic acid as the mobile phase, the volume ratio of acetonitrile-70 mmol·L-1 ammonium acetate and glacial acetic acid is 70:30, the pH of the glacial acetic acid is adjusted to 5.0, and the mobile phase Add 1% (V / V) triethylamine as a tailing agent, the tailing agent further inhibits the effect of silanol and benzalkonium chloride remaining on the surface of the stationary phase, reducing chromatographic peak tailing. ;

[0010] Step 3, then put the sample of disinfectant wipes and mobile phase 25mL into the separation column. The separation column is Eclipse XDB-C18 separat...

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Abstract

The invention discloses a high performance liquid chromatography method for determination of benzalkonium chloride content in antiseptic wipes. The method comprises following steps: (1) weighting a sample of the antiseptic wipes; (2) taking acetonitrile-70 mmol/l ammonium acetate and glacial acetic acid as mobile phase; (3) adding the antiseptic wipe sample and 25 ml mobile phase into a separation column, extracting the sample with mobile phase for 15 min, performing vortex oscillation for 2 min and diluting the mobile phase, wherein a column temperature of the separation column is 40 DEG C, and a determining wavelength is 262 nm; and (4), after dilution, cooling and then transferring the sample into a 100 ml volumetric flask, diluting with mobile phase to 100 ml, and then analyzing. The benzalkonium chloride content is determined by an external standard method, and the benzalkonium chloride content of the antiseptic wipe sample is compared with that of a benzalkonium chloride standard solution.

Description

technical field [0001] The invention relates to a method for determining the content of benzalkonium chloride in disinfectant wet wipes by high performance liquid chromatography. Background technique [0002] Disinfectant wipes are soaked in a disinfectant liquid on non-woven fabrics, which can be used for disinfection or sterilization of skin abrasions and scratches. The disinfectant liquid is mixed with disinfectant and pure water according to the scientific ratio. Before and after meals or without water and soap Wet wipes are a better choice for cleaning your hands and face under certain conditions, and they can also be used for general medical care. Benzalkonium chloride, chemical name dodecyl dimethyl benzyl ammonium chloride (DDBAC), alias geramine, molecular formula C21H38CN, relative molecular mass 340.00. DDBAC is a kind of quaternary ammonium salt compound, which belongs to cationic surfactant, and is often used as a low-efficiency disinfectant for washing and di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 陈妍
Owner PROD QUALITY SUPERVISING & INSPECTING INST OF TAIZHOU CITY
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