Nematocide crystallin gene cry003-148 and application thereof

A crystal protein, nematicide technology, applied in nematicide, application, genetic engineering and other directions, can solve problems such as inability to effectively control agricultural pests, and achieve the effect of wide application value
CN103421097AActive Publication Date: 2013-12-04HUAZHONG AGRI UNIV
2 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2013-12-04

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of microbe genetic engineering, and discloses separation and cloning of nematicidal crystallin gene originated from thuringiensis and biological activity of the nematicidal crystal protein gene on nematode. In the invention, a new insecticidal crystallin gene cry003-148 is obtained by being separated from thuringiensis Sbt003 (the preservation number in China Center for Type Culture Collection is CCTCC NO: M2012168); an encoded product of the gene is a new insecticidal crystallin Cry003-148; and the crystalline has high insecticidal activity on root-knot nematodes in south of China. The invention also comprises preparation of thuringiensis recombinant bacteria BMB1361 which expresses the insecticidal crystallin Cry003-148, the recombinant bacteria is preserved in China Center for Type Culture Collection (CCTCC) and the preservation number is CCTCC NO: M2012057.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the fields of agricultural biological control and agricultural microorganism genetic engineering. It specifically relates to the isolation and cloning of an insecticidal crystal protein gene with nematicide activity and the application of the encoded protein in microbial insecticides and transgenic crops. Background technique

[0002] Bacillus thuringiensis (Bt for short) is a kind of Gram-positive bacteria widely distributed in the environment. It can form endogenous spores in the middle and late stages of its growth and development, and can produce a type of spore along with the formation of spores. Insecticidal Crystal Proteins (ICPs) that have deliberate poisonous activity against various insects are different from Bacillus cereus (Bc) and Bacillus anthracis (Ba). Bt was first isolated from silkworm larvae by Japanese scholars in 1901, and was officially named Bacillus thuringiensis in 1915 (Yu Ziniu, 1990). Currently, mor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More