Specific primer and kit for detecting melon gummy stem blight

Abstract

The invention relates to a specific primer and kit for detecting melon gummy stem blight and a molecular detection method thereof. High specificity of the primer is verified. Didymella bryoniae can be fast and accurately detected by using the primer and the detecting method. The establishment of the detecting system is significant to early-stage fast detection of melon didymella bryoniae, soil didymella bryoniae group distribution detection, and prevention and cure of diseases.

Description

technical field [0001] The invention relates to the field of molecular biology, and develops a primer sequence, a kit and a method capable of rapidly detecting cucurbit blight bacteria. Background technique [0002] Melon blight is caused by the infection of the fungus Ascomycete subphylum Didymella bryoniae (Auessw.) Rehm., also known as Mycosphaerella melonis (Pass.) Chiu et Walker, an asexual stage pathogen It is Ascochyta citrullina Smith, a semi-known fungus subphylum. The fungus of variegated blight survives the winter and summer in the soil with the diseased residue in the form of pycnidia or asthecia, or attached to the surface of seeds, greenhouses, greenhouses, etc. The pathogen can infect watermelons, melons, cucumbers, zucchini, pumpkins, gourds, loofahs and other Cucurbitaceae plants, and is prone to disease in high temperature, high humidity, and poorly ventilated environments, especially in spring greenhouses in Zhejiang and the middle and lower reaches of th...

AI Technical Summary

Problems solved by technology

When the disease is prevalent, a large number of dead vines will appear in the field, seriously affecting the yield and quality of melons

[0003] The early symptoms of melon blight are similar to those of melon wilt, and it is difficult to distinguish them from the symptoms. In addition, the field is prone to compound infection of various pathogens, which makes it more difficult to identify the disease and delays the timing of prevention and control.

However, the traditional method of isolating and identifying pathogenic bacteria to diagnose diseases takes a long time, is cumbersome to operate, has low accuracy, and is difficult to identify potential pathogenic bacteria. Therefore, it is urgent to develop a molecular biology method that can accurately and quickly identify the pathogen method, the incubation period and early onset of melon blight can be diagnosed, detected and identified by pathogenic bacteria, and timely and effective disease control can be carried out to control the prevalence of the disease and reduce economic losses, which has guiding significance for field disease prevention and control

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