Preparation method of silkworm ecdysterone

Abstract

The invention discloses a preparation method of silkworm ecdysterone. The preparation method comprises the following steps of: boiling, refluxing and extracting decumbent bugle herb; clarifying the collected filtrate by using a clarificant and then performing reduced pressure concentration to obtain a solid residue; dissolving the solid residue by using a methanol-ethyl acetate solution; performing reduced pressure concentration to remove an organic solvent and then obtaining a crude product of an ecdysterone extract; separating and purifying the ecdysterone by using macroporous resin and a silica-gel chromatographic column; and finally performing vacuum concentration to obtain an ecdysterone dry product. The ecdysial fluid prepared from the ecdysterone dry product prepared by the preparation method is high in purity and can be used in a quantified mode, the ecdysterone dry product is convenient to store, the storage time is long, and by adopting the ecdysial fluid prepared from the ecdysterone dry product, domestic silkworms grow and develop neatly and are mounted neatly.

Description

technical field

[0001] Embodiments of the present invention relate to the field of processing and manufacturing traditional Chinese veterinary medicines, and more specifically, embodiments of the present invention relate to a preparation method of ecdysterone for preparing molt liquid for silkworms. Background technique

[0002] The manufacturing process of existing molt liquid for silkworms is a traditional production process, and its preparation method is "get 5000g of Herba sinensis, cut into small sections, add water and decoct twice, each time for 1 hour, filter and remove the medicinal residues, merge the filtrate, reduce Concentrate under pressure to about 8000ml, add 25000ml of ethanol, let stand for 24 hours after stirring, filter, recover ethanol from the filtrate under reduced pressure, concentrate the remaining liquid to about 5000ml, add 20000ml of ethanol, stir, let stand for 12 hours, filter, and filtrate Recover ethanol under reduced pressure, concentrate the...