Detection plate and detection kit for in vitro detection of senile dementia

A technology for in vitro detection of senile dementia, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high cost of senile dementia diagnosis and unsuitability for early screening, so as to be suitable for early screening and improve diagnosis The effect of accuracy and low detection cost

Active Publication Date: 2017-02-01
承功(厦门)生物科技有限公司
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  • Abstract
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Problems solved by technology

[0004] Diagnosing Alzheimer's disease currently involves a large number of tests including: medical history, mental status evaluation, physical examination, neurological examination, neuropsychological evaluation, brain MRI, laboratory tests (brain and spinal cord examination); therefore, The current diagnosis of Alzheimer's disease is not only costly, but also not suitable for early screening; it is necessary to develop a method suitable for early screening of early AD patients and normal people in vitro

Method used

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  • Detection plate and detection kit for in vitro detection of senile dementia
  • Detection plate and detection kit for in vitro detection of senile dementia
  • Detection plate and detection kit for in vitro detection of senile dementia

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preparation example

[0068] 1) The detection kit includes, but is not limited to: detection plate, tracer solution, positive control, negative control, sample diluent, washing solution, and chromogenic solution.

[0069] 2) Detection plate preparation:

[0070] Immobilization of biomarker-specific antibodies by indirect coating. First, dilute the rabbit anti-mouse antibody to a concentration of 0.1ug / ml-30ug / ml with PBS buffer, then add 100ul of the antibody dilution to the wells of a 96-well microtiter plate, and incubate at room temperature for 1-4 hours Or incubate at low temperature (4-8°C) for 10-24 hours; wash the microplate wells with PBS+0.1% Tween 20 washing solution for 3-5 times; then, add 200ul of 2% BSA-PBS blocking solution, incubate at room temperature for 1-4 hours, then wash the microplate wells with PBS+0.1% Tween 20 washing solution for 3-5 times;

[0071] Secondly, use PBS buffer to prepare the following 12 biomarker-specific mouse antibody solutions with a concentration of 0...

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Abstract

The invention discloses a detection board and detection kit for in vitro detection of the Alzheimer Disease. The detection board comprises a plurality of detection areas or detection tanks, the detection areas or the detection tanks contain protein of two or more kinds of biomarkers, or corresponding receptor protein or specific antibodies, or DNA primers designed according to corresponding mRNA: Tau, A beta, TNF-alpha, IL-11, IL-6, ANG-2, TRAIL-R4, IL 8, IGFBP 6, ICAM-1, CCL18 and PDGF-BB. The detection board and detection kit for the in vitro detection for the Alzheimer Disease can be used for in vitro blood detection, and are simple, safe and low in cost; the multiple biomarkers are detected at the same time so as to improve the accuracy rate of diagnosis, and the detection board and detection kit for the in vitro detection for the Alzheimer Disease are especially suitable for early screening of the Alzheimer Disease.

Description

technical field [0001] The invention relates to a detection plate for in vitro detection of senile dementia and a detection kit thereof. Specifically, it is a detection plate and detection kit for in vitro blood detection of various biomarkers related to Alzheimer's disease. Background technique [0002] It is estimated that there are 26 million people suffering from senile dementia (English translation: Alzheimer's disease, referred to as: AD) patients in the world. Alzheimer's disease is a very harmful senile mental disease, and the medical field has not yet found a cure for this disease. Although the disease can last up to 20 years, on average AD patients live 8 to 10 years after being diagnosed. The disease usually begins after the age of 60 and increases dramatically with age. Ten percent of Americans over the age of 65 have AD, and 50 percent of those over the age of 85 have AD. Alzheimer's is difficult to recognize in its early stages and can easily be misdiagnose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N33/6896G01N2800/28G01N2800/2814
Inventor 邹潮
Owner 承功(厦门)生物科技有限公司
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