Application OsDRUP1 gene of rice to reinforcement on drought resistance of plant
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A drought resistance, plant technology, applied in applications, plant products, genetic engineering, etc., can solve problems such as complex drought resistance traits in plants
Active Publication Date: 2014-01-15
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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[0006] In recent years, many genes related to plant abiotic stress have been cloned and analyzed, and the mechanism involved in the response to abiotic stress has been preliminarily understood. However, the drought resistance traits of plants are extremely complex, and the genes related to drought resistance need to be further explored and verified.
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Embodiment 1
[0026] Example 1. Differences in temporal and spatial expression of OsDRUP1 gene under drought stress conditions
[0027] Nipponbare, Oryza sativa ssp.japonica (publicly available from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, non-patent literature that has recorded this material is: Yongqing Jiao, Yonghong Wang, Dawei Xue, Jing Wang, Meixian Yan, Guifu Liu , Guojun Dong, Dali Zeng, Zefu Lu, Xudong Zhu, Qian Qian and Jiayang Li(2010) Regulation of OsSPL14by OsmiR156defines ideal plant architecture in rice. Nature Genetics, 42, 541-544);
[0028] 1. Abiotic stress of rice variety Nipponbare and samples of different tissues and organs
[0029] The rice Nipponbare seeds are sterilized, soaked at room temperature for 2 days, and germinated at 37°C for 1 day. After germination, the seeds with consistent germination are selected and sowed on the foam frame of a plastic box, 2 seeds per hole, hydroponic culture before the second leaf, and cultured with ...
Embodiment 2
[0040] Example 2, overexpression of OsDRUP1 gene enhances the drought resistance of transgenic rice
[0041] 1. Overexpression and RNAi expression vector construction
[0042] Primers were designed according to the full-length Nipponbare sequence, and the cDNA was used as a template to amplify the full-length gene of OsDRUP1 with high-fidelity enzyme (same as above). Primers are: F: 5'-GCA CTGCAG ATGGCGGCGCCGGAGAAGGTGTTCG-3' (the underlined part is the restriction site of Pst I); R: 5'-TAA GGATCC TCA GGAAAATGAATACTGATTCCTCCTTGCATGATCTTGGAT-3' (the underlined part is the BamH I restriction site), the amplified product is digested and purified by Pst I and BamH I, and the target DNA fragment (1296bp) is recovered; at the same time, it is expressed by digestion with Pst I and BamH I Vector pCUbi1390 (containing the Ubiquitin1 promoter) (publicly available from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, non-patent documents that have recorded this...
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Abstract
The invention discloses application of an OsDRUP1 gene of rice to reinforcement on drought resistance of a plant. The invention provides application of a protein shown in a sequence 2 in a sequence table to reinforcement on drought resistance of the plant and application of a coding gene of the protein shown in the sequence 2 in the sequence table to reinforcement on drought resistance of the plant. An experimental result of the invention shows that the expression quantity of the OsDRUP1 gene is rapidly increased when the OsDRUP1 gene is stressed by high salt and drought, but the increase extent is low when the OsDRUP1 gene is stressed by low temperature; an OsDRUP1 transcription factor can regulate and control expression of a downstream gene and participate in the plant stress response reaction by combining a GCC box and a DRE in a downstream gene promoter region; overexpression of the OsDRUP1 gene in the rice can improve capacity of resisting to drought stress of the rice to a certain degree. The application disclosed by the invention has very important theoretic and practical significance for improving and reinforcing stress resistance of the rice and accelerating the stress-resistant molecular breeding process.
Description
technical field [0001] The invention relates to the application of rice U box related gene OsDRUP1 in enhancing plant drought resistance in the field of biotechnology. Background technique [0002] Various biotic and abiotic stresses are important factors affecting rice growth and yield. Discovering genes that can improve rice resistance to adversity stress will lay the foundation for genetic engineering to improve new rice varieties. During the long-term evolution process, plants have developed a variety of mechanisms to cope with adversity stress at the molecular, cellular, physiological and biochemical levels. Genetic engineering technology improves the stress resistance of plants by changing the activity of one or several genes. It is carried out at the genetic level, with accuracy and stability, which improves the efficiency of breeding and greatly accelerates the speed of breeding. It is also necessary for us to understand the complex traits and related mechanisms of ...
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