Application OsDRUP1 gene of rice to reinforcement on drought resistance of plant
A drought resistance, plant technology, applied in applications, plant products, genetic engineering, etc., can solve problems such as complex drought resistance traits in plants
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Embodiment 1
[0026] Example 1. Differences in temporal and spatial expression of OsDRUP1 gene under drought stress conditions
[0027] Nipponbare, Oryza sativa ssp.japonica (publicly available from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, non-patent literature that has recorded this material is: Yongqing Jiao, Yonghong Wang, Dawei Xue, Jing Wang, Meixian Yan, Guifu Liu , Guojun Dong, Dali Zeng, Zefu Lu, Xudong Zhu, Qian Qian and Jiayang Li(2010) Regulation of OsSPL14by OsmiR156defines ideal plant architecture in rice. Nature Genetics, 42, 541-544);
[0028] 1. Abiotic stress of rice variety Nipponbare and samples of different tissues and organs
[0029] The rice Nipponbare seeds are sterilized, soaked at room temperature for 2 days, and germinated at 37°C for 1 day. After germination, the seeds with consistent germination are selected and sowed on the foam frame of a plastic box, 2 seeds per hole, hydroponic culture before the second leaf, and cultured with ...
Embodiment 2
[0040] Example 2, overexpression of OsDRUP1 gene enhances the drought resistance of transgenic rice
[0041] 1. Overexpression and RNAi expression vector construction
[0042] Primers were designed according to the full-length Nipponbare sequence, and the cDNA was used as a template to amplify the full-length gene of OsDRUP1 with high-fidelity enzyme (same as above). Primers are: F: 5'-GCA CTGCAG ATGGCGGCGCCGGAGAAGGTGTTCG-3' (the underlined part is the restriction site of Pst I); R: 5'-TAA GGATCC TCA GGAAAATGAATACTGATTCCTCCTTGCATGATCTTGGAT-3' (the underlined part is the BamH I restriction site), the amplified product is digested and purified by Pst I and BamH I, and the target DNA fragment (1296bp) is recovered; at the same time, it is expressed by digestion with Pst I and BamH I Vector pCUbi1390 (containing the Ubiquitin1 promoter) (publicly available from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, non-patent documents that have recorded this...
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