Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of oryza sativa l. OsGA2ox8 protein and coding gene and recombinant vector thereof in enhancing plant drought resistance

A technology for encoding gene and drought resistance, which is applied to rice OsGA2ox8 protein and its encoding gene and recombinant vector to enhance the application field of plant drought resistance

Inactive Publication Date: 2019-11-05
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the OsGA2ox gene mainly focuses on salt stress resistance, and it is not known how it will perform in drought resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of oryza sativa l. OsGA2ox8 protein and coding gene and recombinant vector thereof in enhancing plant drought resistance
  • Application of oryza sativa l. OsGA2ox8 protein and coding gene and recombinant vector thereof in enhancing plant drought resistance
  • Application of oryza sativa l. OsGA2ox8 protein and coding gene and recombinant vector thereof in enhancing plant drought resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Spatiotemporal expression differences of OsGA2ox8 gene under different stress conditions

[0030] Experimental material: Nipponbare (Oryza sativa ssp.japonica) (obtained from the Institute of Crop Science, Chinese Academy of Agricultural Sciences);

[0031] 1. Low temperature, high salinity and drought stress of rice

[0032] The rice Nipponbare seeds are sterilized, soaked at room temperature for 2 days, and germinated at 37°C for 1 day. After germination, the seeds with consistent germination are selected and sowed on the foam frame of a plastic box, 2 seeds per hole, hydroponic before the second leaf, and cultivated with Yoshida nutrient solution after the second leaf . Low temperature (4°C), high salt (150mmol / LNaCl) and osmotic stress (20% PEG) treatments were started at the five-leaf stage. Nipponbare took leaves and roots after 0h, 1h, 3h, 6h, 12h, and 24h of stress treatment.

[0033] 2. Real-time quantitative PCR analysis

[0034] Use TRIZOL reagent to extra...

Embodiment 2

[0050] Overexpression of OsGA2ox8 gene enhances drought resistance of transgenic rice

[0051] 1. Construction of OsGA2ox8 gene overexpression vector

[0052] First, pGWC vector (publicly available from Institute of Crop Science, Chinese Academy of Agricultural Sciences) was digested with restriction endonuclease EmaI1051, linearized and recovered.

[0053] Primers were designed according to the full-length Nipponbare sequence, and the full-length coding region of the OsGA2ox8 gene was amplified using Nipponbare cDNA as a template. At the same time, the linearized adapters of the pGWC vector were added to the 5' and 3' ends. The amplification primers were:

[0054] F: 5′- gcaggctttgacttt ATGGTGGCGATCACGGCGC-3' (the underlined part is the linker sequence);

[0055] R: 5′- gggtctagagacttt TTTCTTCGTCGCGGCCTCATC-3' (the underlined part is the linker sequence); PCR amplification and recovery of the target DNA fragment (1092bp).

[0056] The In-Fusion HD Cloning Kit (Clontech,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of an oryza sativa l. OsGA2ox8 protein and a coding gene and a recombinant vector thereof in enhancing plant drought resistance, and belongs to the technical field of biological gene engineering. The OsGA2ox8 protein has the amino acid sequence shown in SEQ ID NO.1. Under drought, high salt and low temperature stress, along with prolonging of the treatment time and the change of the expression quantity of an OsGA2ox8 gene, and with combination of phenotypic change of overexpressed and CRISPR-knockout oryza sativa l. plants, a result shows that the OsGA2ox8gene in leaves is induced to perform up-regulated expression by drought and cold stress, and is inhibited to perform down-regulated expression in roots by drought and cold stress. In addition, whether in the roots or in the leaves, OsGA2ox8 is induced to perform up-regulated expression by salt stress, but the up-regulated range in the roots is small; with overexpression of the OsGA2ox8 gene in oryza sativa l., the ability of the oryza sativa l. to resist drought stress can be significantly improved.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, and specifically relates to the application of rice OsGA2ox8 protein, its coding gene and recombinant vector in enhancing plant drought resistance. Background technique [0002] Rice (Oryza sativa L.) is a cereal crop with high economic and social value. Together with wheat and corn, it feeds more than half of the world's population. It is the main food crop in my country and is crucial to ensuring food security in my country. However, with the exploration of rice yield potential, there is a sharp contradiction between high input for high yield and sustainable development of resources and environment. In recent years, the increasingly serious salinization of soil or irrigation water has become a major obstacle to high rice yield and further expansion of planting area. [0003] Early studies have shown that the stress tolerance of plants is extremely sensitive to plant hormones, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/82A01H5/00A01H6/46
CPCC12N9/0071C12N15/8273C12N2800/60C12Y114/11013
Inventor 傅彬英王银晓王文生赵秀琴杜丰平徐建龙黎志康
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com