Application of rice osdrup1 gene in enhancing plant drought resistance
A drought resistance, rice technology, applied in applications, plant products, genetic engineering, etc., can solve problems such as complex drought resistance traits in plants
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Embodiment 1
[0025] Example 1. Differences in temporal and spatial expression of OsDRUP1 gene under drought stress conditions
[0026] Nipponbare (Nipponbare, Oryza sativa ssp.japonica) (the public can obtain from the Crop Science Research Institute of the Chinese Academy of Agricultural Sciences, the non-patent literature that has recorded this material is: Yongqing Jiao, Yonghong Wang, DaweiXue, Jing Wang, Meixian Yan, Guifu Liu, Guojun Dong, Dali Zeng, Zefu Lu, Xudong Zhu, Qian Qian and Jiayang Li(2010) Regulation of OsSPL14by OsmiR156defines idealplant architecture in rice. Nature Genetics, 42, 541-544);
[0027] 1. Abiotic stress of rice variety Nipponbare and samples of different tissues and organs
[0028] The rice Nipponbare seeds are sterilized, soaked at room temperature for 2 days, and germinated at 37°C for 1 day. After germination, the seeds with consistent germination are selected and sowed on the foam frame of a plastic box, 2 seeds per hole, hydroponic culture before the se...
Embodiment 2
[0039] Example 2, overexpression of OsDRUP1 gene enhances the drought resistance of transgenic rice
[0040] 1. Overexpression and RNAi expression vector construction
[0041] Primers were designed according to the full-length Nipponbare sequence, and the cDNA was used as a template to amplify the full-length gene of OsDRUP1 with high-fidelity enzyme (same as above). Primers are: F: 5'-GCA CTGCAG ATGGCGGCGCCGGAGAAGGTGTTCG-3' (the underlined part is the restriction site of Pst I); R: 5'-TAA GGATCC TCA GGAAAATGAATACTGATTCCTCCTTGCATGATCTTGGAT-3' (the underlined part is the BamH I restriction site), the amplified product is digested and purified by Pst I and BamH I, and the target DNA fragment (1296bp) is recovered; at the same time, the expression vector is digested with PstI and BamH I pCUbi1390 (contains Ubiquitin1 promoter) (publicly available from Institute of Crop Science, Chinese Academy of Agricultural Sciences, non-patent literature that has recorded this material is:...
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