Method for quercus nuttallii somatic embryogenesis

A technology of somatic cells and Quercus natta, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult establishment, difficult rooting of cuttings, incompatibility of grafting and reproduction, etc., to increase the reproduction coefficient and improve the Effects of regeneration efficiency and reproduction coefficient, normal plant development

Inactive Publication Date: 2014-02-05
江苏艺华园林建设有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, Quercus nata is late in flowering and fruiting, it is difficult to establish a seed garden, the phenomenon of large and small annuals is obvious, it is difficult to take root from cuttings, and there is incompatibility in grafting and propagation, so it is very difficult to produce Quercus nata seedlings on a large scale

Method used

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  • Method for quercus nuttallii somatic embryogenesis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] The culture medium formula of each stage selected in the present embodiment is as follows:

[0085] (1) The formula of bud induction medium is: WPM basic medium, add 0.05mg.L -1 6-BA, 0.06mg.L -1 NAA, 40g.L -1 Sucrose, 7.0 g.L -1Carrageenan, adjust the pH value to 5.7;

[0086] (2) The formula of somatic embryo induction medium is: MS basic medium, plus 0.20 mg. L -1 6-BA, 0.2mg.L -1 NAA, 0.08mg.L -1 2,4-D, 25g.L -1 Sucrose, 6.5g.L -1 Carrageenan, adjust the pH value to 5.7.

[0087] (3) The formula of somatic embryo proliferation medium is: MS basic medium, plus 2.0 mg. L -1 6-BA, 0.01 mg. L -1 NAA, 25g.L -1 Sucrose, 6.5g.L -1 Carrageenan, adjust the pH value to 5.7.

[0088] (4) The formula of somatic embryo maturation medium is: MS basic medium, plus 60g.L -1 Sucrose, 6.5 g.L -1 Carrageenan, adjust the pH value to 5.7.

[0089] (5) The formula of somatic embryo germination medium is: MS medium, plus 12 g. L -1 Sorbitol, 20g.L -1 Sucrose, 6.5 g.L -1...

Embodiment 2

[0091] The culture medium formula of each stage selected in the present embodiment is as follows:

[0092] (1) The formula of bud induction medium is: WPM basic medium, add 0.08mg.L -1 6-BA, 0.05mg.L -1 NAA, 25g.L -1 Sucrose, 6.5 g.L -1 Carrageenan, adjust the pH value to 5.7;

[0093] (2) The formula of somatic embryo induction medium is: MS basic medium, plus 0.16mg. L -1 6-BA, 0.2~0.3mg.L -1 NAA, 0.12 mg.L -1 2,4-D, 40g.L -1 Sucrose, 7.0g.L -1 Carrageenan, adjust the pH value to 5.7.

[0094] (3) The formula of somatic embryo proliferation medium is: MS basic medium, plus 1.8 mg. L -1 6-BA, 0.03 mg.L -1 NAA, 40g.L -1 Sucrose, 7.0 g.L -1 Carrageenan, adjust the pH value to 5.7.

[0095] (4) The formula of somatic embryo maturation medium is: MS basic medium, plus 40g.L -1 Sucrose, 7.0 g.L -1 Carrageenan, adjust the pH value to 5.7.

[0096] (5) The formula of somatic embryo germination medium is: MS medium, plus 8 g. L -1 Sorbitol, 30g.L -1 Sucrose, 7.0 g.L...

Embodiment 3

[0098] The culture medium formula of each stage selected in the present embodiment is as follows:

[0099] (1) The formula of bud induction medium is: WPM basic medium, add 0.06mg.L -1 6-BA, 0.05mg.L -1 NAA, 30g.L -1 Sucrose, 6.8 g.L -1 Carrageenan, adjust the pH value to 5.8;

[0100] (2) The formula of somatic embryo induction medium is: MS basic medium, plus 0.18 mg. L -1 6-BA, 0.25mg.L -1 NAA, 0.1 mg.L -1 2,4-D, 30g.L -1 Sucrose, 6.8g.L -1 Carrageenan, adjust the pH value to 5.8.

[0101] (3) The formula of somatic embryo proliferation medium is: MS basic medium, plus 1.9 mg. L -1 6-BA, 0.02 mg.L -1 NAA, 32g.L -1 Sucrose, 6.8 g.L -1 Carrageenan, adjust the pH value to 5.8.

[0102] (4) The formula of somatic embryo maturation medium is: MS basic medium, plus 45g.L -1 Sucrose, 6.8g.L -1 Carrageenan, adjust the pH value to 5.8.

[0103] (5) The formula of somatic embryo germination medium is: MS medium, add 10g. L -1 Sorbitol, 28g.L -1 Sucrose, 6.8g.L -1 C...

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Abstract

The invention discloses a method for quercus nuttallii somatic embryogenesis. The method comprises a serial of operations, including material selecting, disinfection, induction culture of test-tube seedlings, induction of somatic embryo, enrichment culture, maturation culture and germination of somatic embryo; the culture is performed through culture mediums for induction of somatic embryo, enrichment culture, maturation culture and germination culture. The method has advantages that the propagation coefficient is high, the reproduction time is not limited by the season, the material selection nearly does not hurt the parent, no pest problems occur, and the outstanding performances of original plant can be remained; the method possesses a crucial application value in the production and scientific research of quercus nuttallii nursery stocks.

Description

technical field [0001] The invention relates to a tissue culture method of Quercus nata, in particular to a somatic cell embryogenesis method of Quercus nata. Background technique [0002] Natal oak ( Quercus nuttalli ) is a deciduous tree of the genus Quercus in the family Fagaceae, with an upright trunk, slightly drooping branches, and a tower-shaped crown. The leaves are elliptical, 10-20cm long, 5-13cm wide, dark green on the front, dark green on the back, with tufted hairs, bright red or reddish brown in autumn. Bark gray or brown, smooth. Quercus natta is native to the United States, and its distribution ranges from eastern Texas to western Florida, including Arkansas, Missouri, Oklahoma and southern Tennessee. The introduction practice shows that Quercus nata grows fast, the average tree height of five-year-old can reach 5m, and the diameter of 10-year-old tree is 15-20cm. The leaves start to turn red in early November every year, and the leaves fall in February o...

Claims

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Application Information

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IPC IPC(8): A01H4/00C05G1/06
Inventor 张敏黄利斌陈智敏方炎明蒋泽平蒋春
Owner 江苏艺华园林建设有限公司
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