Peony 4-diphosphate cytidine-2-c-methylerythrosekinase (plispe) gene and its coding product and application

A technology of cytidine diphosphate and methylerythromycin is applied in the field of biosynthesis of organic acids with pharmacologically active components to achieve the effect of increasing the content of terpenoids
CN103571859BInactive Publication Date: 2015-11-25INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
Publication Date
2015-11-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a PLIspE gene and a coded protein and application thereof. The gene is cloned from Paeonia lactiflora by constructing a cDNA library for the first time, so a gap in separation and cloning of a 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase gene from the traditional Chinese medicine--Paeonia lactiflora is filled in. The PLIspE gene provided by the invention has a nucleotide sequence shown in SEQ ID NO. 1, or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of nucleotides of the nucleotide sequence shown in SEQ ID NO. 1, or an allele of the gene or a nucleotide sequence derived from the allele. The protein coded by the gene has an amino acid sequence shown in SEQ ID NO. 2 or a homologous sequence obtained through addition, substitution, insertion or deletion of one or a plurality of amino acids of the amino acid sequence shown in SEQ ID NO. 2. The PLIspE gene can increase the content of metabolites of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol in Paeonia lactiflora through biotechnology and has good application prospects in aspects like quality improvement of the medicinal material Paeonia lactiflora and synthetic biology of active components of Paeonia lactiflora.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and mainly relates to cloning 4-diphosphate cytidine-2-C-methylerythrosekinase gene and its coded product and application by using peony cDNA library, especially relates to the biosynthesis of organic acids with pharmacological active ingredients , terpenoid enzyme gene and its coded product and application, belonging to the field of genetic engineering of medicinal plants. Background technique

[0002] The formation of medicinal plant active ingredients is the product of a unique group of genes in plant secondary metabolic pathways. With the extensive and in-depth study of plant functional genomes, the study of functional genes related to secondary metabolism synthesis of medicinal plants with unique characteristics and broad application prospects has gradually become a research hotspot. It provides a theoretical basis for the biosynthetic pathway and its regulation mechanism and explains the forma...

Claims

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