Group of genes DTH2 for controlling rice heading period and haplotype and application of genes DTH2

A technology of haplotype and heading stage, applied in the field of genetic engineering

Inactive Publication Date: 2014-02-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no gene cloned by QTL has been found to belong to the type that promotes flowering under long-day conditions (Tsuji H, Taoka KI and Shimamoto K(2010) Current Opinion in Plant Biology14:1–8)

Method used

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  • Group of genes DTH2 for controlling rice heading period and haplotype and application of genes DTH2
  • Group of genes DTH2 for controlling rice heading period and haplotype and application of genes DTH2
  • Group of genes DTH2 for controlling rice heading period and haplotype and application of genes DTH2

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Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0031] The construction of embodiment 1DTH2 nearly isogenic line

[0032] 1. QTL detection, backcross and selection of DTH2

[0033] Using the RILs population of Asominori and IR24, our previous study detected multiple loci controlling heading date, one of which, DTH2, contained DTH2-a under natural long-day (NLD) conditions plants heading earlier than plants containing DTH2-i. Using the CSSLs population with Asominori as the background parent and IR24 as the donor parent, we found that CSSL18 has a significantly delayed maturation and stable expression compared with the background parent Asominori, because CSSL18 only inserts IR24 in some fragments compared with the background parent Asominori Therefore, the inserted indica fragment IR24 is the root cause of the extremely late maturity of CSSL18. We crossed CSSL18 with Asominori once, backcrossed 4 times consecutively, and then selfed to construct a CSSL18 / Asominori BC containing 374 individuals 4 f 2 population, from whi...

Embodiment 2

[0039] Fine positioning and map-based cloning of embodiment 2 DTH2

[0040] 1. Identification of heading date phenotypes of two parents

[0041] Heading date refers to the date when the first ear of a single plant is pulled out; days of heading date refers to the number of days from sowing to the first ear of a single plant; extreme late heading single plant refers to the same or later heading date than the parent NIL (DTH2) Single plant; extremely late heading family refers to a family in which most of the individual plants heading in a random family are the same or later than the parent NIL (DTH2). Under NLD conditions in Beijing (Dongmen Net Room, Institute of Crop Science, Chinese Academy of Agricultural Sciences), NIL(DTH2) headed 7.4 days later than Asominori (Table 1, figure 2 C). However, under natural short-day (NSD) conditions in Hainan (Southern Propagation Base of Nanjing Agricultural University in Lingshui, Hainan), there was no significant difference in earing...

Embodiment 3

[0050] Transgenic functional complementation experiment of embodiment 3DTH2

[0051] 1. Construction of genome full-length complementary vector

[0052] According to the predicted candidate gene sequence, using Nipponbare (English name Nipponbare, a rice variety with complete genome sequencing) sequence as a template, primers were designed to sequence the DTH2 genome from Asominori, and it was found that their sequences were consistent in this segment . Then, using the Nipponbare BAC clone OsJNBa33B21 (purchased from the National Gene Research Center of the Chinese Academy of Sciences) as a template, the whole genome of DTH2 was amplified (see Table 4 for primers) to obtain a 7867bp DNA fragment. This fragment was recombined into pCAMBIA1305.1 (a publicly reported and used plasmid from Australia, image 3 ) SmaI (purchased from Fermentas, Canada) site.

[0053] 2. Agrobacterium-mediated genetic transformation of rice

[0054] The obtained recombinant plasmid with correct s...

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Abstract

The invention belongs to the technical field of gene engineering and relates to a group of genes DTH2 for controlling the rice heading period, and a haplotype and application of the genes DTH2. A CDS sequence of the haplotype 3 of the genes DTH2 is presented by SEQ ID NO.6. A transgenosis experiment shows that the genes have a function of allowing the rice heading period to be advanced and proves that the genes are selected during domestication and are from China.

Description

[0001] Case Description [0002] This case is a divisional application of a Chinese invention patent application with the application number 2012100546596 submitted on 2012-03-05, titled "A Group of DTH2 Genes Controlling Heading Date of Rice and Its Haplotype and Application". technical field [0003] The invention belongs to the technical field of genetic engineering, and in particular relates to a group of DTH2 genes for controlling the heading stage of rice, haplotypes and applications thereof. technical background [0004] Heading date is an important agronomic trait and may have been selected during crop domestication (Izawa T. Journal of Experimental Botany 58:3091–3097(2007)). Cultivated rice is currently distributed worldwide over a wide area from latitude 53° north to latitude 40° south (Chang T.T. Euphytica 25, 425-441 (1976)). However, Oryza commonis, the ancestor of cultivated rice in Asia, is mainly distributed in South Asia, Southeast Asia and northern Austra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 万建民吴玮勋陆广文江玲郑晓明钟铮铮
Owner NANJING AGRICULTURAL UNIVERSITY
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