Haplotype of group of genes DTH2 for controlling rice heading period and application of genes DTH2

A technique of haplotype and heading date, applied in the field of genetic engineering

Inactive Publication Date: 2015-05-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no gene cloned by QTL has been found to belong to the type that promotes flowering under long-day conditions (Tsuji H, Taoka KI and Shimamoto K(2010) Current Opinion in Plant Biology14:1–8)

Method used

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  • Haplotype of group of genes DTH2 for controlling rice heading period and application of genes DTH2
  • Haplotype of group of genes DTH2 for controlling rice heading period and application of genes DTH2
  • Haplotype of group of genes DTH2 for controlling rice heading period and application of genes DTH2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0031] Example 1 Construction of DTH2 near-isogenic line

[0032] 1. QTL detection, backcrossing and selection of DTH2

[0033] Using the RILs populations of Asominori and IR24, our previous study detected multiple loci controlling heading date, one of which, DTH2, contained DTH2-a under natural long-day (NLD) conditions. of plants headed earlier than those containing DTH2-i. Using the CSSLs population with Asominori as the background parent and IR24 as the donor parent, we found that CSSL18 was significantly later matured than the background parent Asominori and had stable expression. Compared with the background parent Asominori, CSSL18 only inserted IR24 in some fragments fragment, so the inserted indica fragment IR24 is the root cause of the extremely late ripening of CSSL18. We crossed CSSL18 with Asominori once, backcrossed 4 consecutive times, and then selfed to construct a CSSL18 / Asominori BC containing 374 individuals 4 F 2 population, from which DTH2 was again de...

Embodiment 2

[0039] Example 2 Fine mapping and map-based cloning of DTH2

[0040] 1. Phenotypic identification at heading stage of both parents

[0041] Heading date refers to the date when the first ear of a single plant is drawn; Days of heading date refers to the number of days from sowing to the first ear is drawn from a single plant; Extreme late heading per plant refers to the same or later heading date as the parental NIL (DTH2). Single plant; extremely late heading families refer to families in which the majority of individual headings in a random family are the same or later than the parental NIL (DTH2). Under NLD conditions in Beijing (Dongmen Wang Room, Institute of Crop Science, Chinese Academy of Agricultural Sciences), NIL(DTH2) headed 7.4 days later than Asominori (Table 1, figure 2 C). However, under natural short-day (NSD) conditions in Hainan (Nanfan Base of Nanjing Agricultural University, Lingshui, Hainan), there was no significant difference in heading between the t...

Embodiment 3

[0050] Example 3 Transgenic function complementation experiment of DTH2

[0051] 1. Construction of full-length genome complementary vector

[0052] According to the predicted candidate gene sequence, using the sequence of Nipponbare (English name Nipponbare, a rice variety with complete genome sequencing) as a template, primers were designed to sequence the DTH2 genome derived from Asominori, and it was found that their sequences were consistent in this segment. . Therefore, using the Nipponbare BAC clone OsJNBa33B21 (purchased from the National Genetic Research Center of the Chinese Academy of Sciences) as a template, the whole DTH2 genome was amplified (see Table 4 for primers) to obtain a DNA fragment of 7867 bp. The homologous recombination kit (http: / / bioinfo.clontech.com / infusion / , purchased from Takara, Japan) was used to recombine the fragment into pCAMBIA1305.1 (a publicly reported and used plasmid from Australia, image 3 ) of the SmaI (purchased from Fermentas, C...

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Abstract

The invention belongs to the technical field of gene engineering and relates to a group of genes DTH2 for controlling the rice heading period, and a haplotype and application of the genes DTH2. A CDS sequence of the haplotype 4 of the genes DTH2 is presented by SEQ ID NO.7. A transgenosis experiment shows that the genes have a function of allowing the rice heading period to be advanced and proves that the genes are selected during domestication and are from China.

Description

[0001] Division description [0002] This case is a divisional application for a Chinese invention patent application with the application number 2012100546596 submitted on 2012-03-05, entitled "A Group of DTH2 Genes Controlling the Heading Stage of Rice and Its Haplotypes and Applications". technical field [0003] The invention belongs to the technical field of genetic engineering, in particular to a group of DTH2 gene haplotypes for controlling the heading stage of rice and its application. technical background [0004] Heading date is an important agronomic trait and may be selected during the domestication of crops (Izawa T. Journal of Experimental Botany 58:3091–3097 (2007)). Cultivated rice is currently distributed in a wide area from 53°N to 40°S latitude worldwide (Chang T.T. Euphytica 25, 425–441 (1976)). However, the common wild rice, the ancestor of Asian cultivated rice, is mainly distributed in South Asia, Southeast Asia and northern Australia, and the norther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 万建民吴玮勋陆广文江玲郑晓明钟铮铮
Owner NANJING AGRICULTURAL UNIVERSITY
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