Euphorbiaceae plant active substance, and preparation method and application thereof

An active substance, technology of Euphorbiaceae, applied in the field of preparation of Euphorbiaceae plant active substances and their active ingredients, can solve the problems of animal husbandry loss, food safety concerns, unsatisfactory effect, etc., and achieve the effect of inhibiting proliferation

Inactive Publication Date: 2014-03-12
SCHWEITZER
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, almost all antiviral agents on the market are developed for the purpose of treating human diseases. If these expensive antiviral agents are applied to economic animals, in addition to cost considerations, there may also be food safety concerns. However, if there is no effective vaccine to prevent a certain animal virus, when the herd is infected by the virus, only supportive therapy can be used, but the effect is often unsatisfactory, causing heavy losses in animal husbandry; therefore, it has low side effects The development of safe and effective animal antiviral drugs is an urgent and extremely important matter for the animal health care industry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Euphorbiaceae plant active substance, and preparation method and application thereof
  • Euphorbiaceae plant active substance, and preparation method and application thereof
  • Euphorbiaceae plant active substance, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example Preparation of Euphorbiaceae Plant Active Substances

[0044] 1. Active substances extracted by ethanol (Ethanol)

[0045] Euphorbia neriifolia was collected from Yilan, Taiwan. First, wash the collected diamonds with water and separate the leaves and stems. After standing to dry, weigh them and cut them into slices. Then put the stems and leaves in a brown glass bottle that can protect from light, add 95% (volume percent concentration, 50-99% is acceptable) ethanol to completely immerse the stems and leaves of King Kong in ethanol, and stand for extraction at room temperature until the ethanol changes from transparent and colorless to dark green or dark brown (about 3 -10 days), the ethanol extract was collected, and then concentrated under reduced pressure to remove the ethanol, to obtain the active substances of the stem and leaves of the rhizome extracted with ethanol.

[0046] 2. Active substances extracted by n-hexane (Hexane)

[0047] Dissolve 10 g of th...

Embodiment 2

[0052] Example 2 Preliminary activity analysis of Euphorbiaceae plant active substances extracted with ethanol against PRRSV virus infection

[0053] The active substances in the stem and leaves of the ethanol extracted from Example 1 were first serially diluted twice (500 μg / mL, 250 μg / mL, 125 μg / mL, 62.5 μg / mL, 31.25 μg / mL, 15.625 μg / mL) μg / mL, 7.8125 μg / mL, 3.90625 μg / mL, 1.953125 μg / mL, 0.9765625 μg / mL), followed by treatment of Marc-145 cells (epithelioid cells, derived from monkey kidney cells, derived from mother cells (MA 104 cells) The cloned and obtained PRRSV virus was re-infected to evaluate whether the ethanol-extracted adamantine active substance had the ability to inhibit PRRSV virus infection.

[0054] First, the Marc-145 cells were suspended in MEM medium containing 5% FBS, and then seeded in 96-well cell culture dishes (1.5×10 4 cells / well) at 37°C, 5% CO 2 After culturing cells in a constant temperature incubator for 16-24 hours, remove the supernatant med...

Embodiment 3

[0058] Example 3 Activity analysis of the active substances from the stems of Euphorbiaceae plants extracted with ethanol under different action times against PRRSV virus infection

[0059] The ethanol-extracted adamantium stem active substance (2 μg / mL) obtained in Example 1 was treated with Marc-145 cells for different times and then infected with PRRSV virus to evaluate the relationship between the ethanol-extracted adamantha stem active substance and Marc-145 How long the cells are co-cultured will have the best ability to inhibit PRRSV virus.

[0060] First, the Marc-145 cells were suspended in MEM medium containing 5% FBS and seeded in a 96-well cell culture dish (1.5×10 4 cells / well) at 37°C, 5% CO 2 After culturing the cells in a constant temperature incubator for 16-24 hours, remove the supernatant medium and wash the cells twice with MEM medium containing 1% FBS, then add 2 μg / mL ethanol-extracted active substances from the stems of adamantium (experimental group) ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a Euphorbiaceae plant active substance and a preparation method and application thereof. The active substance is obtained by subjecting a Euphorbiaceae plant to extraction with ethanol; the active substance can be further prepared through extraction with hexane and / or ethyl acetate and / or n-butanol and may be lupeol and / or stigmasterol. The invention further discloses application of the Euphorbiaceae plant active substance in preparation of antiviral drugs for animals.

Description

technical field [0001] The present invention relates to the preparation method of Euphorbiaceae active substances and active ingredients thereof and their application in the field of animal health care, in particular to the preparation method of Euphorbiaceae active substances and active ingredients thereof and their application in prevention and treatment. Application in the treatment of animal virus infection. Background technique [0002] In recent years, the medical application of Chinese herbal medicine has received increasing attention; compared with chemically synthesized drugs, these natural plant extracts have fewer side effects and are relatively safe. It has been pointed out that the extracts of Pittosporaceae and Euphorbiaceae have the ability to inhibit Human Cytomegalovirus (HCMV) (Semple, S.J., Reynolds, G.D., O'Leary, M.C.and Flower, R.L. Screening of Australian medicinal plants for antiviral activity. J Ethnopharmacol 60, 163-172, 1998); in addition, some l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/47A61K31/575A61K31/56A61P31/12
Inventor 郭村勇周盈芳
Owner SCHWEITZER
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap