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A kind of composite bacterial flora for degrading lignin wastewater and its preparation method

A technology of compound flora and lignin, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of difficult biodegradation and low efficiency of biochemical treatment of pulp and paper wastewater.

Inactive Publication Date: 2016-01-06
SOUTH CHINA UNIV OF TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organic substances in the environment, and it At a disadvantage in interspecies competition, traditional biological treatment technologies face great challenges
[0003] Due to the refractory biodegradability of lignin, the biochemical treatment efficiency of pulping and papermaking wastewater is low. With the improvement of the discharge standard of pulping and papermaking wastewater, COD, BOD and toxic substances in pulping and papermaking wastewater can be effectively removed. How to effectively improve the degradation of BOD and COD, reduce the addition of chemicals and operating costs in the subsequent physical and chemical system has become a technical problem that needs to be solved urgently in the existing technology

Method used

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  • A kind of composite bacterial flora for degrading lignin wastewater and its preparation method
  • A kind of composite bacterial flora for degrading lignin wastewater and its preparation method
  • A kind of composite bacterial flora for degrading lignin wastewater and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0022]Pick Agrobacteriumsp., Bacillussp., Enterobacter.Cloacae., Gordoniasp., Pseudomonasputida. Bacteria (Pseudomonasstutzeri.) (isolated from the sludge at the outlet of the paper mill) six species of bacteria 1 ring: transfer them to 20mL nutrient solution (the composition is 1.5g / L beef extract, 1.0g / L L glucose, 5.5g / L tryptone, 3.0g / L yeast powder, pH 6.5, and the rest are water), each type of bacteria was cultured at 27°C for 2 days, and the cultured six kinds of bacteria Inoculate the container containing 300mL of proliferation medium at a volume ratio of 1:9 (bacteria and proliferation medium), culture at 27°C for 2 days, and finally centrifuge at 6000rpm for 15min to obtain the above six species The cells in the logarithmic growth phase of the bacteria were washed with normal saline, dried and refrigerated for later use. Among them, six different bacteria adopt different proliferation medium, the nutrient medium of Agrobacterium, rod-shaped bacteria and Enterobacter...

Embodiment 2

[0028] Pick six rings of bacteria respectively: Agrobacteriumsp., Bacillussp., Enterobacter.Cloacae., Gordoniasp., Pseudomonasputida. ), Pseudomonasstutzeri (Pseudomonasstutzeri.) (separated from the sludge at the outlet of the paper mill) were transferred to a nutrient solution containing 30mL (the composition of which was 2.0g / L beef extract, 1.5g / L glucose, 6.5g / L tryptone, 3.5g / L yeast powder, pH 7.5, and the rest are water), each type of bacteria was cultured at 30°C for 2 days, and the cultured six kinds of bacteria Inoculate them into containers containing 500mL of proliferation medium at a volume ratio of 1:10 (bacteria and nutrient solution), culture them at 35°C for 1 day, and finally centrifuge at 7000rpm for 10min to obtain the above six species The cells in the logarithmic growth phase of the bacteria were washed with normal saline, dried and refrigerated for later use. Among them, six different bacteria adopt different proliferation medium, and the nutrient med...

Embodiment 3

[0034]Pick six rings of bacteria respectively: Agrobacteriumsp., Bacillussp., Enterobacter.Cloacae., Gordoniasp., Pseudomonasputida. ), Pseudomonasstutzeri (Pseudomonasstutzeri.) (separated from the sludge at the outlet of the paper mill) were transferred to 25 mL of nutrient solution (the composition of which was 1.7 g / L beef extract, 2.0 g / L L glucose, 6.0g / L tryptone, 4.0g / L yeast powder, pH 7.0, the rest is water), each kind of bacteria was cultured at 30°C for 2 days, and then the cultured six kinds of bacteria Inoculate them into containers containing 400mL of proliferation medium at a volume ratio of 1:12 (bacteria and nutrient solution), culture them at 35°C for 2 days, and finally centrifuge at 7000rpm for 10min to obtain the above six species The cells in the logarithmic growth phase of the bacteria were washed with normal saline, dried and refrigerated for later use. Among them, six different bacteria adopt different proliferation media, among which the nutrient me...

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Abstract

The invention discloses a composite microflora for degrading lignin wastewater and a preparation method thereof. The method comprises the following steps: first, respectively picking Agrobacterium sp., Bacillus sp., Enterobacter cloacae, Gordonia sp., Pseudomonas putida and Pseudomonas stutzeri for inoculated culture; respectively activating 3-6% of Agrobacterium sp., 3-7% of Bacillus sp., 10-26% of Enterobacter cloacae, 34-45% of Gordonia sp., 16-38% of Pseudomonas putida and 12-23% of Pseudomonas stutzeri by volume, and mixing and casting into wastewater. According to the invention, the degradation efficiency of microbiological treatment of lignin can be effectively improved by the coordinating effect among strains.

Description

technical field [0001] The invention relates to a treatment of lignin wastewater, in particular to a composite bacterial group for degrading lignin wastewater and a preparation method thereof. Background technique [0002] Biological treatment is a commonly used wastewater treatment method at present. This method decomposes and absorbs the pollutants in wastewater through the metabolism of microorganisms, so as to achieve the purpose of pollution control. Compared with other methods, biological treatment has low cost, high efficiency, easy operation, and most importantly, no secondary pollution. Therefore, it has been widely used in wastewater treatment. With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organic substances in the environment, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/40C12R1/38C12R1/01C02F103/28
Inventor 陈元彩宋文哲胡勇有
Owner SOUTH CHINA UNIV OF TECH
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