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siRNA molecule composition, and applications thereof in treatment of hypertrophic scars

A technology of hypertrophic scar and composition, which is applied in the fields of molecular biology and gene therapy to achieve good therapeutic effect and the effect of promoting apoptosis of hypertrophic scar fibroblasts

Inactive Publication Date: 2014-03-19
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this document discloses that the application of siRNA to wounds can promote healing and reduce scar formation during the healing process. Wound treatment and scar treatment are two completely different concepts, involving different physiological processes. Treatment of established hypertrophic scars has not been reported

Method used

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  • siRNA molecule composition, and applications thereof in treatment of hypertrophic scars
  • siRNA molecule composition, and applications thereof in treatment of hypertrophic scars
  • siRNA molecule composition, and applications thereof in treatment of hypertrophic scars

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Experimental program
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Effect test

Embodiment 1

[0047] 1 Experimental materials

[0048] 1.1 Active ingredients of siRNA

[0049] TGF-β1 siRNA molecule: sense strand: 5′-ccccggaggugauuuccaucuacaa-3′, antisense strand: 5’-uuguagauggaaaucaccuccgggg-3′;

[0050]COX-2 siRNA molecule: sense strand: 5′-gucuuuggucuggugccuggucuga-3′, antisense strand: 5’-ucagaccaggcaccagaccaaagac-3′;

[0051] Drug carrier: peptide (HKP), a polymer of histidine and lysine;

[0052] STP705 (siRNA cocktail): The above-mentioned TGF-β1 siRNA molecules, COX-2 siRNA molecules and HKP are formed into nanoparticles, wherein the copy number ratio of TGF-β1 siRNA molecules and COX-2 siRNA molecules is 1:1, and the two siRNA molecules are mixed with The weight ratio of HKP is 1:4. During the treatment, the siRNA concentration of 20 μg / ml was injected into the local lesion.

[0053] The above drugs were provided by Suzhou Shengnuo Biological Co., Ltd.

[0054] 1.2 Antibody

[0055] Rabbit anti-human α-actin (α-SMA, alpha smooth muscle Actin) monoclonal a...

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Abstract

The invention relates to a siRNA molecule composition, and applications thereof in treatment of hypertrophic scars. The siRNA molecule composition comprises siRNA molecules, whose nucleotide sequence comprises a) 5'-ccccggaggugauuuccaucuacaa-3', and 5'-uuguagauggaaaucaccuccgggg-3'; and siRNA molecules, whose nucleotide sequence comprises b) 5'-gucuuuggucuggugccuggucuga-3', and 5'-ucagaccaggcaccagaccaaagac-3'. The siRNA molecule composition is capable of promoting apoptosis of hypertrophic scar fibroblast in vitro, and possesses excellent therapeutic effect on hypertrophic scars of animal models.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and gene therapy, in particular, the application of siRNA molecules in the treatment of hypertrophic scars. Background technique [0002] The skin is the largest organ of the human body and has functions such as protection, immunity, and excretion, among which the most important function is to protect and maintain the homeostasis of the internal environment. When the integrity of the skin is greatly damaged, the homeostasis in the body becomes unbalanced, and external invasion factors easily enter the body, directly threatening the survival of the individual. In the process of species evolution, the scar healing mode of wounds appeared. This healing method can restore the integrity of the skin as quickly as possible, greatly improving the survival rate of the species. Therefore, scar is an indispensable product of wound healing in human skin. [0003] However, in the process of wound ...

Claims

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Application Information

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IPC IPC(8): C12N15/11A61K48/00A61P17/02
Inventor 周佳刘凯黄晓璐李青峰
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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