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Composition and method for killing drug-resistance aspergillus fumigatus

A technology of drug-resistant Aspergillus fumigatus and composition, applied in the field of microorganisms

Active Publication Date: 2014-04-02
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to control the IPA caused by drug-resistant Aspergillus fumigatus has become a clinical problem, and the development of new effective drugs is a way to solve this problem. However, the development of new effective drugs is a long process, and it is not easy to deal with the current severe drug resistance. situation
If a certain drug can cooperate with traditional antifungal drugs to make the latter play a stronger role in inhibiting Aspergillus fumigatus, it will also be of great help to alleviate the pressure of drug resistance, but no similar reports have been seen so far

Method used

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  • Composition and method for killing drug-resistance aspergillus fumigatus
  • Composition and method for killing drug-resistance aspergillus fumigatus
  • Composition and method for killing drug-resistance aspergillus fumigatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Materials

[0017] 1. Drugs Amphotericin B (Amresco, lot number 1397893), cinnamaldehyde (Sigma, lot number V900539). Dissolve them in 100% dimethyl sulfoxide to make storage solutions with concentrations of 1280 μg / ml and 102400 μg / ml, and store them at -20°C after aliquoting.

[0018] 2. The strains were collected from 7 Aspergillus fumigatus strains collected in the First Affiliated Hospital of Guangxi Medical University from September 2012 to May 2013. The quality control strain was Candida parapsilosis ATCC22019, which was donated to the Bacteria Laboratory of the First Affiliated Hospital of Guangxi Medical University.

[0019] 3. Reagents and equipment Potato glucose medium PDA (Beijing Luqiao), MOPS (Sigma), RPMI-1640 powder (Gibco), dimethyl sulfoxide (Sigma), sterile 96-well cell culture plate (Corning, USA), biochemical culture Box (Changzhou Weijia Instrument Manufacturing Co., Ltd., model: SPX-250), biological safety cabinet (Suzhou Purification Equipm...

Embodiment 2

[0028] This example is the result of the detection of drug-resistant Aspergillus fumigatus activity in Example 1.

[0029] 1. Drug susceptibility results The MICs of AMB to 7 Aspergillus fumigatus strains were all 2 μg / ml.

[0030] 2. MIC value The MIC of cinnamaldehyde and AMB alone and in combination on 7 strains of Aspergillus fumigatus 50 、MIC 90and MICG (Table 1), after statistical analysis, the MICG of AMB 单用 and MICG 联合 compared to Z =-2.414, P 单用 and MICG 联合 compared to Z =-2.384, P <0.05.

[0031] Table 1 Cinnamaldehyde and AMB alone and in combination, the MIC against Aspergillus fumigatus (μg / ml)

[0032] (n=7)

[0033]

[0034] 3. Cumulative antibacterial percentage curve Concentration-cumulative antibacterial percentage curve of cinnamaldehyde and AMB alone and in combination ( figure 1 )

[0035] 4. FIC distribution of cinnamaldehyde combined with AMB The combined application of cinnamaldehyde and AMB acted on 7 Aspergillus fumigatus strains, th...

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Abstract

The invention provides a composition and a method for killing drug-resistance aspergillus fumigatus, and belongs to the technical field of biology. The method for killing drug-resistance aspergillus fumigatus comprises the following steps: preparing a medicine; collecting a strain; preparing a spore suspension; performing a drug sensitivity test and a combining drug susceptibility test. By virtue of the steps, the minimum inhibitory concentration (MIC) of amphotericin B and cinnamyl aldehyde can be obtained, and the effect of killing drug-resistance aspergillus fumigatus can be achieved by utilizing the medicinal combinations of amphotericin B and cinnamyl aldehyde with different concentrations.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to aspergillus fumigatus biofilm, and in particular relates to a composition for killing drug-resistant aspergillus fumigatus and a method for killing drug-resistant aspergillus fumigatus. Background technique [0002] Invasive pulmonary aspergillosis (IPA) caused by Aspergillus fumigatus is a common and fatal opportunistic infectious disease in immunocompromised patients such as hematological malignancies, organ transplantation, and AIDS, with a mortality rate as high as 30-100%. Drug resistance is an important cause of treatment failure. A clinical study tested 1385 Aspergillus fumigatus strains in IPA patients and found that the mortality rate of IPA patients resistant to azoles was 88%, while the mortality rate of those infected with sensitive strains was 48%. %. In recent years, the resistance rate of Aspergillus fumigatus has gradually increased. In 1999, azole-resistant Asp...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/10A61K31/11
Inventor 陈一强黄宏孔晋亮温汉春
Owner GUANGXI MEDICAL UNIVERSITY
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