Ultralow temperature cryopreservation and recovery method of Micropterus salmoides sperm

A technology of ultra-low temperature freezing and largemouth bass, which is applied to the preservation of human or animal bodies, germ cells, and vertebrate cells, etc. It can solve the problems of decreased growth rate, decreased ability to resist diseases, lack of directional breeding, etc., and achieve the operation time Short, less damage, good induction effect

Active Publication Date: 2015-07-08
SUZHOU MUNICIPAL SHENHANG ECO TECH DEV CO LTD
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AI Technical Summary

Problems solved by technology

However, due to the lack of directional selective breeding due to domestication of wild species at the time of introduction, largemouth bass have experienced varying degrees of germplasm degradation over time, mainly manifested in early sexual maturity age, reduced growth rate, and reduced disease resistance. , it is imperative to carry out the breeding of largemouth bass, and to breed strains with fast growth and good quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The choice of broodstock: normal morphology, good growth, disease-free, and sexually mature original species or elite species.

[0016] Sperm collection: during the breeding period of largemouth bass, 800UI / kg intraperitoneal injection was used to induce spermatogenesis, 36h later, the semen was collected in a clean mortar pre-cooled in a refrigerator at 4℃.

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Abstract

The invention provides an ultralow temperature cryopreservation and recovery method of Micropterus salmoides sperm, which comprises the following steps: (1) collecting seminal fluid, (2) selecting anti-freezing liquid, (3) diluting and balancing sperms, (4) refrigerating sperms, (5) unfreezing and recovering sperms. The method provided by the invention has the advantages of short operation time, good induction effect, little toxicity effect, little damage degree, high survival rate, etc., and has important theoretical meanings and application values for germplasm preservation, genetic breeding, sustainable culture and disease control and other aspects. The invention also provides a purpose of ultralow temperature cryopreservation and recovery method of Micropterus salmoides sperm.

Description

Technical field [0001] The invention relates to the technical field of sperm freezing, in particular to a method for freezing and storing largemouth bass sperm. Background technique [0002] my country is a large aquaculture country. After the rapid development of conventional breeding species, the breeding of special aquatic products has developed rapidly. Largemouth bass has many advantages such as fast growth, strong adaptability, less disease, and easy fishing. Its meat is delicious and nutritious, and it is quite popular with farmers and consumers. Since its introduction in the 1980s, large-scale industries have been formed in Guangdong, Zhejiang and Jiangsu. However, due to the introduction of wild breeders and domestication and lack of targeted breeding, largemouth bass has experienced varying degrees of germplasm degradation over time, mainly manifested in earlier sexual maturity, decreased growth speed, and decreased disease resistance. , It is imperative to carry out ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02C12N5/076
Inventor 徐海华王荣泉宣云峰
Owner SUZHOU MUNICIPAL SHENHANG ECO TECH DEV CO LTD
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