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Strain of degrading herbicide 2,4-dichlorophenoxyacetic acid and application thereof

A kind of technology of dichlorophenoxyacetic acid and herbicide, applied in the fields of environmental engineering and microbial engineering

Active Publication Date: 2014-04-23
西林县招商中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, a strain of Pseudomonas sp. Ld1 was isolated in the patent "A 2,4-Dichlorophenoxybutyl Pesticide Residue Degrading Bacteria and Its Production Agent" (CN101402927B), which can make 2 , The residue of 4-dichlorophenoxyacetic acid butyl ester is reduced by more than 70%, but there is no patent report on the degradation of 2,4-dichlorophenoxyacetic acid by Cupriavidus campinensis

Method used

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  • Strain of degrading herbicide 2,4-dichlorophenoxyacetic acid and application thereof
  • Strain of degrading herbicide 2,4-dichlorophenoxyacetic acid and application thereof
  • Strain of degrading herbicide 2,4-dichlorophenoxyacetic acid and application thereof

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Embodiment 1

[0024] The invention provides a method for separating and screening the herbicide 2,4-dichlorophenoxyacetic acid-degrading bacterial strain BJ71.

[0025] 10g of surface soil samples at a distance of 5 to 29 cm below the surface were collected from the wheat field soil where the herbicide 2,4-dichlorophenoxyacetic acid had been applied for many years in Mulin Town, Shunyi District, Beijing, and added to 500mg / L 2,4-dichlorophenoxy Carry out enrichment culture in 100ml MSM liquid medium of acetic acid; the condition of enrichment culture is 30°C 150rpm shaker shaking culture for 7 days, transfer 5% culture solution to fresh medium with the same composition, and culture under the same conditions , repeat this process 4 times; after that, take 1ml of the 4th culture solution and dilute it, spread it on the MSM solid medium containing 500mg / L 2,4-dichlorophenoxyacetic acid and incubate at 30°C for 3-5 days; Inoculate the single colony grown on the selective medium containing 2,4-d...

Embodiment 2

[0042] Bioremediation of 2,4-dichlorophenoxyacetic acid-contaminated soil using Cupriavidus campinensis BJ71 strain.

[0043] Preparation of BJ3-71 inoculum solution: Select a ring of colony from the plate and insert it into LB liquid medium containing 500 mg / L 2,4-dichlorophenoxyacetic acid, the volume of the shake bottle is 40% (v / v), Activate overnight at 150rpm at 30°C; pipette 500μl of the activated bacteria solution, transfer it to the fresh medium with the same composition as above, and cultivate to OD under the same conditions 600 0.6 or so; 5000g centrifugation for 5 minutes to collect the bacteria, washed twice with 0.85% normal saline, suspended in a small amount of normal saline for later use.

[0044] Soil treatment: divided into 4 treatment groups. A. Unsterilized soil group: unsterilized soil was treated with 500ppm2,4-D; B. Unsterilized soil+BJ71 treatment group: unsterilized soil was treated with 500ppm2,4-D, then inoculated with BJ71 bacterial solution; C ....

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Abstract

The invention relates to a strain of degrading herbicide 2,4-dichlorophenoxyacetic acid and an application thereof, belonging to the technical fields of environmental engineering and microbial engineering. The strain cupriavidus campinensis of degrading herbicide 2,4-dichlorophenoxyacetic acid is named BJ71, with collection number of CCTCC (China Center for Type Culture Collection) M2014006. The strain has the capacity of growing with 2,4-dichlorophenoxyacetic acid as the unique carbon source and energy, and can obviously reduce the concentration of 2,4-dichlorophenoxyacetic acid in the environment. Experiments prove that the strain has more than 92% of 2,4-dichlorophenoxyacetic acid degradation rate in 21 days, and the experiments show that the strain can be applied to bioremediation of 2,4-dichlorophenoxyacetic acid polluted soil.

Description

technical field [0001] The invention belongs to the technical fields of environmental engineering and microbial engineering, and in particular relates to a bacterial strain degrading herbicide 2,4-dichlorophenoxyacetic acid and its application in bioremediation of polluted soil. Background technique [0002] 2,4-Dichlorophenoxyacetic acid (2,4-D) is a highly active plant growth regulator, which can promote plant growth and induce callus formation at low concentrations; but As a phenoxycarboxylic acid herbicide in high concentration, it is widely used in the control of broad-leaved weeds in cereal crops (especially wheat fields and corn fields), pastures and lawn grasses. It has been widely used for 70 years since industrialized production in the 1940s. There are nearly 100 herbicide products in my country's pesticide market, and the top 20 products in the use area account for about 75% of the total area of ​​chemical weeding in the country's farmland. 2,4 -Dichlorophenoxyace...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 赵德刚韩丽珍
Owner 西林县招商中心
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