Fish collagen peptide and preparation method thereof

A technology of fish collagen peptide and collagen, which is applied in the direction of fermentation, etc., can solve the problems of complex process, high risk, residue, etc., and achieve the effect of simple production process and high economic benefit

Inactive Publication Date: 2014-04-30
DALIAN MEILE BIOLOGICAL TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages: (1) Extracting collagen peptides from the skin and bones of livestock will cause problems such as pigs and cattle that often have foot-and-mouth disease or mad cow disease. Extracting collagen peptides from the skin and bones of livestock has a high Dangerous issues (2) The economic benefits of extracting collagen peptides from livestock skin and bones are low (3) The process is complicated, the product quality is poor, and the obtained collagen products have residual problems of toxic and harmful reagents, which cannot be well processed Removal of heavy metals in deep-sea fish cannot control product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The preparation method of fish collagen peptide is realized according to the following steps:

[0014] (1) Add 0.4 kg of fish skin to 0.8 kg of protease for enzymolysis, and then inactivate the enzyme to obtain crude collagen extract;

[0015] (2) Add 1.2 kg of net activated carbon to the crude collagen extract obtained in the step (1) and stir for decolorization and deodorization, and filter to obtain the collagen serum;

[0016] (3) Flow the collagen serum obtained in step (2) through 1.1 kg of arsenic-removing resin, then wash away the arsenic resin exchange column with purified water, and collect the eluate;

[0017] (4) Evaporating and concentrating the eluate from step (3), and spray-drying to obtain fish skin collagen with a molecular weight of 2000-3000 Daltons.

Embodiment 2

[0019] The preparation method of fish collagen peptide is realized according to the following steps:

[0020] (1) Add 0.6kg of fish skin to 1.2kg of protease for enzymolysis, and then inactivate the enzyme to obtain crude collagen extract;

[0021] (2) Add 0.9 kg of net activated carbon to the crude collagen extract obtained in the step (1) and stir for decolorization and deodorization, and filter to obtain the collagen serum;

[0022] (3) Flow the collagen serum obtained in step (2) through 0.8 kg of arsenic-removing resin, then wash away the arsenic resin exchange column with purified water, and collect the eluate;

[0023] (4) Evaporating and concentrating the eluate from step (3), and spray-drying to obtain fish skin collagen with a molecular weight of 2000-3000 Daltons.

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Abstract

The invention discloses a fish collagen peptide which mainly comprises fish skin, protease, net activated carbon and arsenic removal resin. The preparation method comprises the following steps: (1) adding the fish skin into the protease for performing enzymolysis, inactivating the protease, and obtaining a crude extract of the collagen; (2) adding the net activated carbon into the crude extract of the collagen obtained in the step (12) for stirring, discoloring and removing the fishy smell, and filtering to obtain collagen liquor; (3) allowing the collagen liquor obtained in the step (2) to flow through the arsenic removal resin, washing an arsenic removal resin exchange column by using purified water, and collecting the eluant; and (4) evaporating and concentrating the eluant in the step (3), performing spray drying, and obtaining the fish collagen peptide with the molecular weight of 2000-3000Daltons. The fish collagen peptide is extracted by utilizing various fishes, is safe, sanitary and harmless and is high in economic benefits and simple in preparation process.

Description

technical field [0001] The invention relates to a preparation method of a nutritional product, in particular to a preparation method of a protein peptide. Background technique [0002] Collagen peptide is an important functional protein, which is a hydrolyzed product of collagen, and has the characteristics of low molecular weight and easy absorption. The raw materials of collagen peptide products come from the skin and bones of livestock, as well as fish. Its disadvantages: (1) Extracting collagen peptides from the skin and bones of livestock will cause problems such as pigs and cattle that often have foot-and-mouth disease or mad cow disease. Extracting collagen peptides from the skin and bones of livestock has a high Dangerous issues (2) The economic benefits of extracting collagen peptides from livestock skin and bones are low (3) The process is complicated, the product quality is poor, and the obtained collagen products have residual problems of toxic and harmful rea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
Inventor 韩广权
Owner DALIAN MEILE BIOLOGICAL TECH DEV
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