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Gastric cancer detection serum/plasma miRNA (micro ribonucleic acid) marker and application thereof

A marker and biomarker technology, applied in the fields of biotechnology and oncology, can solve the problems of misdiagnosis or missed diagnosis, lack of human experience, improper sampling of endoscopy and exfoliated cells, etc., and achieve the effect of high sensitivity and simple operation.

Active Publication Date: 2014-05-07
NANJING MEDICAL UNIV
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Problems solved by technology

[0003] The diagnosis of gastric cancer is generally based on clinical symptoms, combined with signs and endoscopic examination, X-ray barium meal examination, B-mode ultrasonography, CT examination, magnetic resonance imaging MRI examination and exfoliative cytology examination, but these methods are not effective in early diagnosis. There are certain defects: Imaging examinations have certain limitations in the diagnosis of early gastric cancer, improper endoscopic examination and exfoliated cell examination, or insufficient human experience will lead to misdiagnosis or missed diagnosis
Whether the expression of these miRNAs in serum / plasma is consistent with that in gastric cancer tissue, and whether it can be used as a specific marker for gastric cancer for the diagnosis of gastric cancer remains to be further studied

Method used

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  • Gastric cancer detection serum/plasma miRNA (micro ribonucleic acid) marker and application thereof
  • Gastric cancer detection serum/plasma miRNA (micro ribonucleic acid) marker and application thereof
  • Gastric cancer detection serum/plasma miRNA (micro ribonucleic acid) marker and application thereof

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Embodiment 1

[0021] Embodiment 1: the collection of verification sample, epidemiological investigation and clinical data

[0022] Collection of tissue samples: Part of the tumor tissue was cut from the tumor lesion, and the corresponding adjacent normal tissue was cut at least 5 cm away from the lesion, and immediately stored in liquid nitrogen.

[0023] Collection of serum / plasma samples: Each patient and control provided 5 mL of fresh blood, and the upper layer of serum / plasma was collected after centrifugation and stored at -80°C. The controls were all healthy persons.

[0024] All patients had been pathologically diagnosed by senior pathologists, and had no other tumors before inclusion in this study, and had not been treated with radiation or chemical drugs. Both cases and controls use a unified health status questionnaire, and field epidemiological investigations of cases and controls are conducted in a face-to-face manner. The survey content included general demographic characteri...

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Abstract

The invention discloses a gastric cancer detection serum / plasma miRNA (micro ribonucleic acid) marker and an application thereof. The biomarker is any one or more out of miR-148a, miR-142, miR-26a and miR-195, wherein miR-148a is as shown in SEQIDNO:1; miR-142 is as shown in SEQIDNO:2; miR-26a is as shown in SEQIDNO:3; miR-195 is as shown in SEQIDNO:4. According to the marker and the application thereof, a gastric cancer patient tissue miRNA chip expression profile is combined with a serum / plasma miRNA chip expression profile for use for the first time, and the screened miRNA marker is tumor-specific, high in sensitivity and applicable to large-scale screening, and gastric cancer can be simply diagnosed.

Description

technical field [0001] This patent belongs to the field of biotechnology and oncology, relates to the application of serum / plasma microRNA (microRNA, referred to as miRNA), and discloses a serum / plasma miRNA for detecting gastric cancer and its application. Background technique [0002] Gastric cancer is one of the most common tumors in the world. Although the overall incidence of gastric cancer in the world has shown a downward trend in recent years, its absolute incidence is still huge, and its morbidity and mortality rank fourth and third among malignant tumors, respectively. two. In my country, the morbidity and mortality of gastric cancer rank first among all kinds of tumors. There are more than 300,000 newly diagnosed patients every year, two-thirds of which are advanced gastric cancer, and the age of onset has a clear trend of younger. Early gastric cancer is mostly asymptomatic or only mildly symptomatic. When the clinical symptoms are obvious, the disease is alrea...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张正东王美林康美云刘桑储海燕仝娜吴冬梅赵庆洪龚伟达
Owner NANJING MEDICAL UNIV
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