A kind of preparation method of Duosuike fermented tea and the prepared Duosuike fermented tea
A technology for multi-spike and fermented tea, applied in the field of fermented tea, can solve the problems of poor retention and different, and achieve the effects of excellent effect, soft taste, and abundant space utilization.
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Embodiment 1
[0033] Embodiment 1: the preparation of Duo Suike fermented tea
[0034] In this example, the following steps are adopted to prepare Duosuike health-care fermented tea:
[0035] 1) Activation of strains: Aspergillus oryzae 40013, Curtobacterium sp. 13-13 (preservation number: CCTCC No. M2014009), Bacillus pumilus purchased from China Type Culture Collection Center (Bacillus pumilus) 13-03 (preservation number is CCTCC No.M2014008) were inoculated in PDA liquid medium (potato 20%, glucose 2%, natural pH), 28 ℃, activated culture until the concentration of each bacterial solution was 106cfu / mL .
[0036] 2) Mixing of strains: Use a straw to draw 100mL, 100mL and 200mL from the activated bacterial liquid of Aspergillus oryzae 40013, non-spore-free Pumilus and Bacillus pumilus respectively, mix them evenly in a ratio of 1:1:2, and set aside.
[0037] 3) Initial fertilization pile: Take 500g of Duosuike green tea into a sterile triangular flask, inoculate 400mL of activated mixed...
Embodiment 2
[0070] Embodiment 2: Comparison of the brewing effect of Duosuike fermented tea
[0071] Take 5g of Duosuike fermented tea obtained according to the method of Example 1 and 5g of original tea (before fermentation) and brew with boiling water for 15 minutes respectively, and compare their brown color and mouthfeel.
[0072] image 3 Shown is the comparison result of the brown color after brewing of the fermented tea of Duosuike fermented tea produced by the method of Example 1 of the present invention and the brown color of the original tea (before fermentation). Brown (B), distinctly different from the light green (A) of the original tea. The prepared tahoko fermented tea tastes soft and mellow, with the sweetness of tahoko itself.
Embodiment 3
[0073] Embodiment 3: the preparation of Duo Suike fermented tea
[0074] The difference between this example and Example 1 is that only Aspergillus oryzae 40013 is used as the fermenting bacteria to prepare Duosuike fermented tea, and other processes are the same as in Example 1.
[0075] Measure the content of various ingredients in the Duosuike fermented tea prepared by the present embodiment as shown in Table 2:
[0076] Table 2 Summary of main components in Duosuike fermented tea
[0077]
[0078] It can be seen from Table 2 that the contents of catechins, theaflavins and flavonoids in the fermented tea prepared in this example are slightly lower than those in Example 1.
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