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Strain for producing haematochrome and method for producing haematochrome

A technology of red pigment and strain, applied in the field of microbial engineering, can solve the problems of poor pigment stability, high cost, complex production process, etc., and achieve the effect of strong antioxidant ability and α-glucosidase inhibitory ability, and cost saving.

Inactive Publication Date: 2014-05-14
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the natural pigments currently used are extracted from natural animal and plant materials, which have the disadvantages of limited resources, complicated production process, high cost, low extraction rate and poor pigment stability, which are far from meeting the needs of the market.

Method used

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  • Strain for producing haematochrome and method for producing haematochrome
  • Strain for producing haematochrome and method for producing haematochrome
  • Strain for producing haematochrome and method for producing haematochrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of strains

[0045] After sterilizing the potato solid medium with natural pH (200g potato, 18g glucose, 18g agar and 1000mL distilled water), pour it into a plate, cool it overnight, check that it is not infected, insert YCPP-01 strain, and cultivate it at 28°C for 7 A fresh strain of YCPP-01 was obtained 2 days later.

Embodiment 2

[0046] Example 2 Microbiological characteristics and identification of strains

[0047] The physiological and biochemical characteristics of the YCPP-01 fresh bacterial strain obtained in the above-mentioned Example 1 are as follows: the colony is round, white, and the edge is entire; The hyphae are white and fluffy and grow rapidly; the culture medium changes from colorless to purple, such as figure 1 shown. The specimen slices were prepared by the Medan water immersion method, and the individual morphology was observed under a microscope: hyphae without septa, branched, conidia, conidiophores branched or not branched. The small conidia are oval and without septum; the mycelium cells of the large conidia produce red pigment, filamentous fungi with conidia, and the mycelium is white and fluffy. figure 2 Further shows the morphological figure of the above-mentioned YCPP-01 bacterial strain under the scanning electron microscope (wherein figure 2 a is a 10000 times magnif...

Embodiment 3

[0049] Example 3 Effects of medium, temperature and pH value on the cultivation of strain YCPP-01 (Lecanicillium.sp)

[0050] The YCPP-01 strain was cultured on potato solid medium, Sabouraud solid medium and Chapeauer solid medium (refer to Table 1 for each medium formula), and 0.5×0.5cm seed bacteria were inserted on each plate, pH The value is set to five pH values ​​of 5, 6, 7, 8, and 9, and the temperature is set to five values ​​of 20°C, 25°C, 28°C, 30°C, and 35°C, and the hyphae are observed at the above pH values ​​and temperatures Growth status and timing of red pigment production.

[0051] Table 1: Description of each medium composition

[0052]

[0053] The experimental results showed that the strain YCPP-01 could grow on the above three mediums and produce pigments, but the pigments produced in Sabouraud medium were relatively small. The strains on the potato medium grew best, with large colonies, dense mycelia, and many conidia; the strains on the Sabouraud...

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PUM

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Abstract

The invention provides a strain for producing haematochrome. The strain is separated from porphyridium, is lecanicillium lecanii, is named as YCPP-01 (Lecanicillium.sp), and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation date is September 16th, 2013 and the preservation number is CGMCC No.8168. Furthermore, the invention further provides a method for producing the haematochrome by adopting the strain YCPP-01 (Lecanicillium.sp). The strain YCPP-01 (Lecanicillium.sp) is used for producing the haematochrome so as to overcome the disadvantage of taking animals and plants as raw materials to prepare the haematochrome; the industrial large-scale production can be realized.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and relates to a strain producing red pigment and a method for producing red pigment by using the strain. Background technique [0002] Pigment is a substance that has dyeing effect, can dye organic substances or is used for color tracing, and can be used to improve the color of articles and foods. Therefore, pigments are widely used in food, cosmetics and pharmaceuticals. [0003] Pigments are usually divided into synthetic pigments and natural pigments according to their sources. Synthetic pigments have the advantages of bright color, strong tinting strength, stable properties and low price, and are widely used. However, synthetic pigments not only have no nutritional value but also have varying degrees of toxicity, and some even have the effect of causing cancer or inducing chromosomal mutations. In recent years, the safety of synthetic pigments has attracted attention from all...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02C09B61/00C12R1/645
Inventor 许伟程文邵荣颜秀花陈立根余晓红陈洪兴
Owner YANCHENG INST OF TECH
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