Tissue culture rapid propagation method of salvia miltiorrhiza bge

A technology of purple flower salvia, tissue culture and rapid propagation, applied in the field of plant reproduction, can solve the problems of affecting yield and quality, degeneration of salvia species, etc.

Inactive Publication Date: 2014-05-21
WEIFANG VOCATIONAL COLLEGE
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Problems solved by technology

However, the species of Salvia miltiorrhiza is seriously degraded, which affects the yield and quality. It is necessary to purify and rejuvenate, and to promote new varieties. Pu...

Method used

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Embodiment Construction

[0017] Below in conjunction with embodiment, further set forth the present invention. In the following detailed description, certain exemplary embodiments of the invention are described by way of illustration only. Needless to say, those skilled in the art would realize that the described embodiments can be modified in various different ways, all without departing from the spirit and scope of the present invention. Accordingly, the description is illustrative in nature and not intended to limit the scope of the claims.

[0018] The rapid propagation method of Salvia miltiorrhiza by tissue culture comprises the following steps:

[0019] Step 1, explant treatment, select the stem of Salvia miltiorrhiza with axillary buds as the explant, cut the explant with a length of 2-3cm, 2.5cm is the best; place the explant in a container filled with detergent water In the bottle, keep shaking and cleaning for 13-17 minutes, 15 minutes is the best, then rinse the explants repeatedly with ...

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Abstract

The invention discloses a tissue culture rapid propagation method of salvia miltiorrhiza bge. The method comprises the steps of selecting explants, treating the explants, carrying out inoculation induction culture, carrying out rooting culture, carrying out bottle cap-opening hardening seedling for 3-5 days, and planting; after the method is used for inoculation, the explants are high in survival rate; plantlets germinated from axillary buds induced in a culture medium are robust, the nodes are obvious, new leaves are normal to spread and grow, the new leaves of the salvia miltiorrhiza bge are emerald green, have no formation of the callus, and have no generation of vitrification and browning; after the method is used for propagating the salvia miltiorrhiza bge, the salvia miltiorrhiza bge is high in survival rate, and the batch and rapid propagation can be realized.

Description

technical field [0001] The present invention relates to plant propagation technology, in particular to a kind of propagation technology of salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza, also known as red ginseng, purple salvia miltiorrhiza, red root, etc., is a perennial herb of the Lamiaceae. Salvia miltiorrhiza is a traditional bulk medicinal material in my country. It is used as medicine with dried roots and rhizomes. It has the effects of dispelling blood stasis and relieving pain, promoting blood circulation, clearing heart and eliminating troubles, nourishing blood and calming the nerves. It is mainly used clinically to treat cardiovascular diseases such as angina pectoris, coronary heart disease, and myocardial infarction. . [0003] Salvia miltiorrhiza is mainly produced in Shandong, Anhui, Henan, Shaanxi and other places, and purple danshen is widely cultivated. Many rural areas in my country rely on planting Salvia miltiorrhiza as a cash c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 雷世俊房师梅王洪波丁雪珍
Owner WEIFANG VOCATIONAL COLLEGE
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