A kind of artificial disease-causing method of in vitro inoculation of tobacco bacterial wilt

Abstract

The invention discloses an artificial disease attack method for tobacco bacterial wilt in-vitro inoculation. The method comprises the following steps: (1) preparing main appliances for inoculation; (2) performing tobacco seedling cultivation; (3) preparing a bacteria solution for inoculation; (4) collecting in-vitro leaves; (5) treating the in-vitro leaves; (6) inoculating pathogenic bacteria; (7) treating after inoculation. The method has the advantages that 1, the appliances used in the method are simple, the inoculation and disease attack process is short in time, the occupied space is small, the method is simple and convenient, high-difficulty technical operation is not needed, and the working efficiency is high; 2, small tobacco leaves are utilized, tobacco seedling cultivation preparation is rapid and feasible, the disease attack symptoms and field symptoms are consistent, the tobacco seedlings are clearly and easily identified, the reproducibility is high, the local inoculation and disease attack conditions are easily controlled, and standardization of the technical method is easily realized.

Description

technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is an artificial disease-causing method for in vitro inoculation of tobacco bacterial wilt. Background technique [0002] In the study of plant disease control and prevention, the research on many basic issues or important theoretical issues cannot be separated from the basic means of artificial inoculation. The artificial disease of plant diseases can be inoculated on living plants, and can also be inoculated on isolated plant organ materials. Although the research results of using living plants to inoculate the pathogenesis can better reflect the original appearance of the host-bacteria interaction, inoculation on living organisms often requires more time and space, and consumes more energy and financial resources. However, it is difficult to control local conditions; while the use of isolated plant organ materials to inoculate the disease often requires le...

AI Technical Summary

Problems solved by technology

Although this inoculation method system is very effective, there are also some obvious disadvantages: 1) The operation technique is difficult. To accurately infiltrate or inject the bacterial solution into the thinner leaf tissue whose thickness is almost equal to the diameter of the needle, not only Well-trained operators also have relatively low operating efficiency; 2) The results of inoculation mainly manifest as local necrosis of tissues near the inoculation point, which is somewhat different from the symptoms of systemic infection of the disease

In the high-paced contemporary work, it is often required that the applied technical methods are simple and convenient to work with high efficiency, high throughput and high accuracy. The above-mentioned disadvantages obviously make it difficult for the current in vitro tobacco inoculation method to meet the requirements.

[0005] The main symptom of tobacco bacterial wilt is wilting, and it can also cause necrosis of the conduction system and its surrounding tissues. The conduction tissue of the leaf is distributed on the leaf veins, and the necrosis of the conduction system and its surrounding tissues can lead to changes in the appearance of the leaf veins. Brown to black necrosis, this symptom feature should be used in artificial inoculation, but so far there is no such clear symptom expression, application and supporting technology in artificial inoculation practice

Images