Artificial disease attack method for tobacco bacterial wilt in-vitro inoculation

Abstract

The invention discloses an artificial disease attack method for tobacco bacterial wilt in-vitro inoculation. The method comprises the following steps: (1) preparing main appliances for inoculation; (2) performing tobacco seedling cultivation; (3) preparing a bacteria solution for inoculation; (4) collecting in-vitro leaves; (5) treating the in-vitro leaves; (6) inoculating pathogenic bacteria; (7) treating after inoculation. The method has the advantages that 1, the appliances used in the method are simple, the inoculation and disease attack process is short in time, the occupied space is small, the method is simple and convenient, high-difficulty technical operation is not needed, and the working efficiency is high; 2, small tobacco leaves are utilized, tobacco seedling cultivation preparation is rapid and feasible, the disease attack symptoms and field symptoms are consistent, the tobacco seedlings are clearly and easily identified, the reproducibility is high, the local inoculation and disease attack conditions are easily controlled, and standardization of the technical method is easily realized.

Description

Technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is an artificial method for inoculating tobacco bacterial wilt in vitro. Background technique [0002] In the research of plant disease control and prevention, many basic issues or important theoretical issues are inseparable from the basic method of artificial inoculation. The artificial onset of plant diseases can be inoculated on living plants, or inoculated on isolated plant organ materials. The results of research on the incidence of disease by inoculation of live plants can better reflect the nature of host-pathogen interaction, but inoculation in vivo often requires more time, space and space, and consumes more energy and financial resources, and in many cases However, it is more difficult to control local conditions; however, using isolated plant organ materials to inoculate disease usually requires less time and space, and is simple and easy to control. L...

AI Technical Summary

Problems solved by technology

Although this inoculation method system is very effective, there are also some obvious disadvantages: 1) The operation technique is difficult. To accurately infiltrate or inject the bacterial solution into the thinner leaf tissue whose thickness is almost equal to the diameter of the needle, not only Well-trained operators also have relatively low operating efficiency; 2) The results of inoculation mainly manifest as local necrosis of tissues near the inoculation point, which is somewhat different from the symptoms of systemic infection of the disease

In the high-paced contemporary work, it is often required that the applied technical methods are simple and convenient to work with high efficiency, high throughput and high accuracy. The above-mentioned disadvantages obviously make it difficult for the current in vitro tobacco inoculation method to meet the requirements.

[0005] The main symptom of tobacco bacterial wilt is wilting, and it can also cause necrosis of the conduction system and its surrounding tissues. The conduction tissue of the leaf is distributed on the leaf veins, and the necrosis of the conduction system and its surrounding tissues can lead to changes in the appearance of the leaf veins. Brown to black necrosis, this symptom feature should be used in artificial inoculation, but so far there is no such clear symptom expression, application and supporting technology in artificial inoculation practice

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