A rapid method for evaluating the efficacy of citrus canker agents

Abstract

The invention belongs to the technical field of citrus disease prevention and control, in particular to a method for rapidly evaluating the efficacy of citrus canker medicaments. A method for quickly evaluating the drug efficacy of citrus canker agents, comprising the following steps: (1) preparation of main utensils for inoculation; (2) collection of diseased leaves of citrus canker; (3) collection of healthy isolated leaves of citrus; (4) canker Disposal of diseased leaves; (5) Disposal of detached leaves; (6) Inoculation of pathogens; (7) Disposal of chemicals after inoculation and (8) Disposal of chemicals. The method of the present invention uses simple utensils, no high-difficulty technical operation, and is simple and easy; skips the preparation stage of the bacterial solution, shortens the time for inoculating the germs, and takes up little space; the symptoms of the disease are consistent with those in the field, and are easy to identify, and the difference in the control effect of the medicament is significant. The onset conditions of inoculation are easy to control, and it is easy to realize the standardization of technical methods.

Description

technical field [0001] The invention belongs to the technical field of citrus disease prevention and control, in particular to a method for rapidly evaluating the efficacy of citrus canker medicaments. Background technique [0002] In the pesticide efficacy test in the field of citrus pest control, in addition to the field spraying prevention experiment, due to the relatively good control of the environment, the method of simulating the occurrence of the disease in the laboratory is also widely used, and the basic method of artificial inoculation is required for relevant research. . In vivo inoculation or in vitro inoculation can be selected as the method of artificial disease pathogenesis. The advantage of in vivo inoculation is that it can better reflect the true appearance of the pathogenic bacteria. The disadvantage is that the test cycle is long, takes up more space and resources, and is often in a multi-factor environment. It is difficult to control local conditions; ...

AI Technical Summary

Problems solved by technology

In recent years, the citrus industry has developed vigorously, and many varieties sensitive to citrus canker have been planted and promoted in large quantities. The prevention and control of citrus canker is also urgent. However, due to the uneven planting and cultivation techniques and experience of citrus planting enterprises and individuals, it has brought great challenges to its control. greater difficulty

In addition to the traditional copper preparations, antibiotics, and allicin for the prevention and treatment of citrus canker, the market is also flooded with a large number of new citrus canker control agents. To evaluate whether these agents are effective, the traditional agent screening, the field test period is too long, and the efficiency low, the environment is complex, and the conditions are difficult to control; the specific operation of laboratory drug screening requires a good background in botany and microbiology

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