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A kind of artificial pathogenic method of in vitro inoculation of tobacco blight

A technology for blight and tobacco, applied in the field of agricultural technology and biology, to achieve the effect of good reproducibility, less space occupation and simple equipment

Active Publication Date: 2015-10-21
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the disease mainly harms the stem base of tobacco plants, the inventors have found that R. solani can also infect the upper leaves of tobacco and cause clear necrotic lesions. This pathogenic characteristic can be used in artificial inoculation. Tobacco blight can infect the favorable characteristics of leaves and cause disease, apply it in the practice of artificial disease and establish supporting technologies

Method used

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  • A kind of artificial pathogenic method of in vitro inoculation of tobacco blight
  • A kind of artificial pathogenic method of in vitro inoculation of tobacco blight
  • A kind of artificial pathogenic method of in vitro inoculation of tobacco blight

Examples

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Effect test

Embodiment 1

[0033] An artificial pathogenic method of in vitro inoculation of tobacco blight, inoculating tobacco blight bacterial strain Rs-1 on the in vitro leaves of tobacco seedling variety Yunyan 87, operates according to the following steps:

[0034] 1. Preparation of main equipment: Use a common petri dish with a diameter of 15cm as the equipment for inoculation, clean and disinfect it for later use.

[0035] 2. Cultivation of tobacco seedlings: Cultivate the tobacco seedling variety Yunyan 87 according to the conventional method, and manage the conventional water and fertilizer until the length of the leaves of the tobacco seedlings is consistent with the diameter of the petri dish in step 1 (the length of the leaves is about 14cm), and then it can be used.

[0036] 3. Preparation of inoculated mycelium: transplant the strain Rs-1 to the center of the PSA medium plate. The components of the PSA medium are: 200 grams of potatoes, 20 grams of sucrose, 18 grams of agar, and 1000 mL of...

Embodiment 2

[0043] An artificial pathogenesis method for in vitro inoculation of tobacco blight, inoculating tobacco solanum blight strain Rs-1 on the in vitro leaves of tobacco seedling variety Yunyan 87, the operation steps are carried out according to step 1 to step 7 of embodiment 1, wherein The operation in step 7 is different from the operation in step 7 of Example 1. In step 7 of Example 1, the culture temperature after inoculation is maintained at 25-27 ° C, and in step 7 of this embodiment) the culture temperature after inoculation is maintained at 19 ~ 21°C. The results also showed as Figure 3 ~ Figure 5 The same clear leaf necrosis symptoms, but the lesion enlargement rate is slower than the speed of embodiment 1, and the necrotic lesion diameter is 1.7cm in 72 hours after inoculation.

Embodiment 3

[0045] Adopt a kind of artificial pathogenesis method of in vitro inoculation of tobacco blight of the present invention, inoculate tobacco blight bacilli strain Rs-1 on the in vitro leaves of tobacco seedling variety Yunyan 87, the operation steps are according to step 1) to step 7 of embodiment 1 ) operation implementation, wherein the operation in step 3) is different from the operation in step 3) of Example 1, the cultivation time of the inoculum hyphae in step 3) of Example 1 is 48 hours, and the step 3) inoculation of this example The culture time of somatic mycelium is 96 hours. The results also showed as Figure 3 ~ Figure 5 The same clear leaf necrosis symptoms, but the lesion enlargement rate is slower than the speed of embodiment 1, and the necrotic lesion diameter is 1.9cm in 72 hours after inoculation.

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Abstract

The invention discloses an artificial infection method for in-vitro inoculation of tobacco bacterial wilt. The method comprises the following steps: (1) preparing a main appliance for inoculating; (2) cultivating tobacco seedlings; (3) preparing inoculation mycelia; (4) collecting in-vitro leaves; (5) treating the in-vitro leaves; (6) inoculating bacteria; (7) treating after inoculating. The artificial infection method has the advantages that 1, the used appliance is simple and the inoculating infection process has short time and few occupied space field; disease symptoms are clear and easy to identify, and the repeatability is good; (2) the implementation and operation are simple, easy and convenient; the partial condition of the inoculating infection is easy to control and the standardization of a technical method is easy to realize.

Description

technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is an artificial disease-causing method for in vitro inoculation of tobacco blight. Background technique [0002] In the study of plant disease control and prevention, the research on many basic issues or important theoretical issues cannot be separated from the basic means of artificial inoculation. The artificial disease of plant diseases can be inoculated on living plants, and can also be inoculated on isolated plant organ materials. Although the research results of using living plants to inoculate the pathogenesis can better reflect the original appearance of the host-bacteria interaction, inoculation on living organisms often requires more time and space, and consumes more energy and financial resources. However, it is difficult to control local conditions; while the use of isolated plant organ materials to inoculate the disease often requires less time ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 张君成黄柳谭金乐陈江凝
Owner GUANGXI UNIV
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