Identification method of morel fungus anti-white mold
An identification method and technology of white mold disease, applied in the field of identification of Morchella fungus resistance to white mold disease, can solve the problems of mutual infection and poor accuracy, and achieve the effects of improving accuracy, easy operation, easy identification and statistics
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Embodiment 1
[0026] (1) Preparation of the inoculation vessel: sterilize the glass plate, filter paper, distilled water and tweezers by high pressure steam at 121°C for 30 minutes, fix the sterile air-permeable sealing film into a cylindrical model with fine wire, and sterilize for later use.
[0027] (2) Preparation for inoculation of pathogenic bacteria: the inoculation of pathogenic bacteria Paecilomyces penicillatus was collected from the Soil and Fertilizer Research Institute of Sichuan Academy of Agricultural Sciences, and the pathogenic bacteria were activated and transferred to PDA medium (200g potatoes, 20g glucose, 18g agar, 1000ml distilled water), at 28°C Cultivate in the incubator for 5 days, and use the colony as the inoculation material.
[0028] (3) Morchella fruiting body preparation: cover the aseptic sealing film model in step (1) on the freshly formed Morchella fruiting body, put on sterile gloves, collect and dispose of 2d healthy Morchella species, and put Pull up the...
Embodiment 2
[0032] According to embodiment one step (1) (2) operation implementation. Morel fruiting body preparation: cover the aseptic sealing film model in step (1) on the newly formed Liumei morel fruiting body, put on sterile gloves, collect and dispose of healthy Liumei morel species for 2 days, put sheep Pull up the fruiting body and the soil part of the tripe fungus, remove the soil at the bottom of the stipe with a clean brush, and bring it back indoors in a clean container for later use. Afterwards, implement the steps (4) (5) of the implementation case, and the results are as follows: A1, B1: the performance after 48 hours of inoculation; A2, the performance of B2 after 96 hours of inoculation; C1, C2: the performance of the control after 48 hours and 96 hours . The proportion of lesions 96 hours after inoculation was 8.6%±0.7%.
Embodiment example 3
[0034] Follow steps (1) (2) of the implementation case 1 to implement. Morel fruiting body preparation: cover the aseptic sealing film model in step (1) on the freshly formed morel sporocarp, put on sterile gloves, collect and dispose of healthy morel species for 4 days, put sheep Pull up the fruiting body and the soil part of the tripe fungus, remove the soil at the bottom of the stipe with a clean brush, and bring it back indoors in a clean container for later use. Afterwards, implement the steps (4) (5) of the implementation case, and the results are as follows: A1, B1: the performance after 48 hours of inoculation; A2, the performance of B2 after 96 hours of inoculation; C1, C2: the performance of the control after 48 hours and 96 hours . The proportion of lesions 96 hours after inoculation was: 7.1%±1.0%.
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