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Identification method of morel fungus anti-white mold

An identification method and technology of white mold disease, applied in the field of identification of Morchella fungus resistance to white mold disease, can solve the problems of mutual infection and poor accuracy, and achieve the effects of improving accuracy, easy operation, easy identification and statistics

Active Publication Date: 2020-05-19
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the method of field inoculation for resistance identification, the diseased fruiting bodies are infected with each other, and the accuracy of identification is poor.

Method used

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  • Identification method of morel fungus anti-white mold
  • Identification method of morel fungus anti-white mold
  • Identification method of morel fungus anti-white mold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of the inoculation vessel: sterilize the glass plate, filter paper, distilled water and tweezers by high pressure steam at 121°C for 30 minutes, fix the sterile air-permeable sealing film into a cylindrical model with fine wire, and sterilize for later use.

[0027] (2) Preparation for inoculation of pathogenic bacteria: the inoculation of pathogenic bacteria Paecilomyces penicillatus was collected from the Soil and Fertilizer Research Institute of Sichuan Academy of Agricultural Sciences, and the pathogenic bacteria were activated and transferred to PDA medium (200g potatoes, 20g glucose, 18g agar, 1000ml distilled water), at 28°C Cultivate in the incubator for 5 days, and use the colony as the inoculation material.

[0028] (3) Morchella fruiting body preparation: cover the aseptic sealing film model in step (1) on the freshly formed Morchella fruiting body, put on sterile gloves, collect and dispose of 2d healthy Morchella species, and put Pull up the...

Embodiment 2

[0032] According to embodiment one step (1) (2) operation implementation. Morel fruiting body preparation: cover the aseptic sealing film model in step (1) on the newly formed Liumei morel fruiting body, put on sterile gloves, collect and dispose of healthy Liumei morel species for 2 days, put sheep Pull up the fruiting body and the soil part of the tripe fungus, remove the soil at the bottom of the stipe with a clean brush, and bring it back indoors in a clean container for later use. Afterwards, implement the steps (4) (5) of the implementation case, and the results are as follows: A1, B1: the performance after 48 hours of inoculation; A2, the performance of B2 after 96 hours of inoculation; C1, C2: the performance of the control after 48 hours and 96 hours . The proportion of lesions 96 hours after inoculation was 8.6%±0.7%.

Embodiment example 3

[0034] Follow steps (1) (2) of the implementation case 1 to implement. Morel fruiting body preparation: cover the aseptic sealing film model in step (1) on the freshly formed morel sporocarp, put on sterile gloves, collect and dispose of healthy morel species for 4 days, put sheep Pull up the fruiting body and the soil part of the tripe fungus, remove the soil at the bottom of the stipe with a clean brush, and bring it back indoors in a clean container for later use. Afterwards, implement the steps (4) (5) of the implementation case, and the results are as follows: A1, B1: the performance after 48 hours of inoculation; A2, the performance of B2 after 96 hours of inoculation; C1, C2: the performance of the control after 48 hours and 96 hours . The proportion of lesions 96 hours after inoculation was: 7.1%±1.0%.

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Abstract

The invention discloses a method for identifying anti-white mold performance of morchella esculenta. The method can solves the problems of mutual infection of pathogenesis sporophore in a resistance identification method, poor identification accuracy and poor efficiency during field inoculation. The method comprises the following steps: 1) preparing an inoculation vessel; 2) preparing inoculation pathogenic pathogen; 3) preparing morchella esculenta sporophore; 4) processing the morchella esculenta sporophore; 5) inoculating the white mold pathogen; and 6) processing the white mold pathogen after inoculation. The method has the advantages of simple operation, small occupation space, controllable inoculation environment, simplenes and rapidity, and high resistance identification efficiency; and also has the advantages that the disease symptom is consistent with that in field, disease spot is single, which is convenient for identification and statistics, reappearance is good, compared with the resistance identification of field inoculation nature disease, mutual infection of the disease sporophore can be effectively avoided, and the identification accuracy is increased.

Description

technical field [0001] The invention is related to the identification method of morel fungus against white mold. Background technique [0002] Morel (Morchella) is a kind of precious edible and medicinal fungus, which is rich in essential amino acids, polysaccharides and terpenoids, and has active effects such as enhancing immunity, anti-oxidation, anti-tumor, protecting liver and kidney. With the expansion of the cultivation scale of hickory chick, pests and diseases have become an important factor limiting the development of hickory chick industry. White mold is the most common disease of Morchella, which is caused by Paecilomyces penicillatus. Since the country’s extensive commercial cultivation in 2012, the incidence of white mold has gradually increased. Therefore, it is necessary to strengthen the prevention and control of Morchella white mold Controlling and speeding up the selection and breeding of resistant varieties to Morchella white mold is an inevitable require...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 陈诚卢代华张鸿王剑伏荣桃龚学书罗曦李星月
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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