Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Volvariella volvacea strain 0229 molecule specific detection markers and detection method using the same

A technology for detecting markers and detection methods, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. , the detection time is short, the effect of speeding up the research and development work

Active Publication Date: 2014-06-04
SHANGHAI ACAD OF AGRI SCI
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Our country has a wide area of ​​straw mushroom cultivation, and its output once ranked fifth among edible fungi, but the variety is single. After testing the mating gene types of the main cultivated straw mushroom strains, it was found that most of the existing strains originated from wild grasses in the 1970s. Mushroom isolate strain V23, obtained through systematic breeding, has a relatively narrow genetic background, but has great differences in agronomic traits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Volvariella volvacea strain 0229 molecule specific detection markers and detection method using the same
  • Volvariella volvacea strain 0229 molecule specific detection markers and detection method using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] DNA extraction:

[0024] Inoculate the PDA plate medium with the strain of straw mushroom, culture it at 32°C for 5 days, scrape the surface mycelia and put it into a sterile centrifuge tube, store it in a -70°C refrigerator, and then use the improved CTAB method to extract genomic DNA (the method is briefly described as follows: Grind the freeze-dried mycelia into powder, add 2×CTAB extract preheated at 65°C, dispense into 2mL centrifuge tubes, keep warm at 65°C for 45min, and shake gently occasionally; centrifuge at 4°C, 12000rpm for 20min, and take the supernatant Add an equal volume of chloroform: isoamyl alcohol (24:1) mixture, mix gently, and centrifuge at 12000rpm for 20min at 4°C after 15min. Take the supernatant and transfer it to a new centrifuge tube, add 2 / 3 volume -20°C Pre-cooled isopropanol, shake gently, centrifuge at 4°C, 8000rpm for 10min after 5min. Discard the supernatant, wash the pellet with 75% ethanol (containing 10mmol / L NaAc) three times, centr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to volvariella volvacea strain 0229 molecule specific detection markers and a detection method using the same. The volvariella volvacea strain 0229 molecule specific detection markers are specific primers and sequences of the specific primers comprise F: CTTTCAGGAAGAGCCCAGC and R: TGAAGGGCTTGAAGGAGAAA. The detection method comprises the following steps of inoculating a PDA flat medium with a volvariella volvacea strain, carrying out culture at a temperature of 32 DEG C for 5 days, collecting surface hyphae by scraping, putting the surface hyphae into a sterile centrifuge tube, carrying out preservation at a temperature of -70 DEG C in a refrigerator, extracting a genome DNA by an improved CTAB method to obtain a DNA, carrying out PCR amplification by the DNA as a template and specific detection marker primers as amplimers to obtain a PCR amplification product, and carrying out electrophoresis detection. The volvariella volvacea strain 0229 molecule specific detection markers have the advantages of short detection time and high accuracy, are used for molecular marker-based identification of volvariella volvacea strain 0229, are conducive to volvariella volvacea breeding development and accelerate research and development of a novel volvariella volvacea variety.

Description

technical field [0001] The invention belongs to the field of detection marks and detection methods of straw mushroom strains, and in particular relates to a molecular-specific detection mark of straw mushroom strain 0229 and a detection method thereof. Background technique [0002] Straw mushroom (Volvariella volvacea) cultivation originated in China, has a cultivation history of more than 300 years so far, and is widely cultivated in southern my country. Straw mushrooms are delicious, not only rich in nutritional value, but also have an important role in health care, and are deeply loved by consumers. Straw mushroom is a high-temperature cultivated edible fungus. Compared with other edible fungi, its remarkable feature is that its growth rate is extremely fast. It usually only takes 2 weeks from sowing to harvesting. Its annual output has always ranked first in my country's edible fungi industry. Top ten. [0003] Our country has a wide area of ​​straw mushroom cultivation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/11C12Q1/68
Inventor 汪虹王莹陈明杰鲍大鹏冯爱萍
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com