The invention discloses a modified barley flour for preventing chicken virus hepatitis and a preparation method thereof. The modified barley flour is prepared from the following raw materials in parts by weight: 700-800 parts of barley, 8-12 parts of malic acid, 3-4 parts of purslane, 4-5 parts of dyers woad leaf, 2-3 parts of honeysuckle flower, 45-47 parts of white fish meal, 5-7 parts of minced garlic, 23-25 parts of grape seed pulp, 12-16 parts of corn flour, 7-8 parts of locust bean dreg, 4-5 parts of chicken bouillon, 3-4 parts of persimmon vinegar, 18-21 parts of blood flour, 14-16 parts of potato flour, 3-4 parts of peanut oil, 0.5-0.8 part of active dry yeast, 7-8 parts of bitter gourd, 12-13 parts of celery, 13-17 parts of carboxymethyl starch and 2-3 parts of table salt. The modified barley flour can reinforce the immunological competence of chicken, prevent chicken virus hepatitis, reduce the sickness rate of the chicken, relieve the disease development state, ensure safe and healthy growth of the chicken, increase the weight gain rate and improve the farming yield.